The details of the immunogen for this antibody are not available.
This antibody blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.
Our Abpromise guarantee covers the use of ab25478 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|Flow Cyt||Use 2µg for 106 cells. ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.|
|IHC-Fr||Use at an assay dependent concentration. Acetone fixed.|
|IP||Use at an assay dependent concentration.|
|Functional Studies||Use at an assay dependent concentration.
(In vivo and in vitro depletion of CD8a+ cells): Use at an assay dependent dilution.Fractionation of CD8a+ T cells: Use at an assay dependent dilution.
Flow Cytometry analysis of BALB/c mouse splenocytes labeling CD8 alpha with ab25478 at 1 μg/106 cells, and Rat Anti-Mouse CD4-UNLB, followed by Mouse Anti-Rat IgG2a-AF647 and Mouse Anti-Rat IgG2b-AF488.