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The details of the immunogen for this antibody are not available.
This antibody blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.
Our Abpromise guarantee covers the use of ab25499 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
ab129719-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|IHC-Fr||Use at an assay dependent concentration.
|IP||Use at an assay dependent concentration.|
|Functional Studies||Use at an assay dependent concentration.
In vivo and in vitro depletion of CD8α+ cells. Fractionation of CD8α+ T cells.
Flow Cytometry analysis of C57Bl/6 splenocytes labeling CD8 alpha with ab25499 at 0.25 µg (solid line), and Rat IgG2a (Allophycocyanin) isotype control at 0.25 µg (dashed line).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"