Anti-CD8 alpha antibody [53-6.7] (Allophycocyanin/Cy7 ®) (ab25456)

Overview

  • Product nameAnti-CD8 alpha antibody [53-6.7] (Allophycocyanin/Cy7 ®)
    See all CD8 alpha primary antibodies
  • Description
    Rat monoclonal [53-6.7] to CD8 alpha (Allophycocyanin/Cy7 ®)
  • ConjugationAllophycocyanin/Cy7 ®. Ex: 650nm, Em: 774nm
  • Specificityab25456 recognises a chain of the murine CD8 heterodimer.
  • Tested applicationsSuitable for: Flow Cyt, IHC-Fr, IP, Functional Studiesmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • General notesThis product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.


    This antibody blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.

Properties

Applications

Our Abpromise guarantee covers the use of ab25456 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.2µg for 106 cells.
IHC-Fr Use at an assay dependent concentration.

Acetone fixed.

IP Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration.

In vivo and in vitro depletion of CD8α+ cells. Fractionation of CD8α+ T cells.

Target

  • FunctionIdentifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
  • Involvement in diseaseDefects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
  • Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • alpha polypeptide (p32) antibody
    • CD8 antibody
    • CD8 antigen alpha polypeptide antibody
    • CD8 antigen alpha polypeptide (p32) antibody
    • CD8a antibody
    • CD8A antigen antibody
    • CD8A molecule antibody
    • CD8A_HUMAN antibody
    • Leu2 antibody
    • Leu2 T lymphocyte antigen antibody
    • Ly3 antibody
    • LYT3 antibody
    • MAL antibody
    • OKT8 T cell antigen antibody
    • OTTHUMP00000160760 antibody
    • OTTHUMP00000160764 antibody
    • OTTHUMP00000203528 antibody
    • OTTHUMP00000203721 antibody
    • p32 antibody
    • T cell antigen Leu2 antibody
    • T cell co receptor antibody
    • T-cell surface glycoprotein CD8 alpha chain antibody
    • T-lymphocyte differentiation antigen T8/Leu-2 antibody
    • T8 T cell antigen antibody
    • T8/Leu-2 T-lymphocyte differentiation antigen antibody
    see all

References for Anti-CD8 alpha antibody [53-6.7] (Allophycocyanin/Cy7 ®) (ab25456)

ab25456 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"