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The details of the immunogen for this antibody are not available.
In vivo and in vitro treatment with ab25176 effectively depletes CD8 alpha+ cells. ab25176 also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.
Our Abpromise guarantee covers the use of ab25176 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.
In vivo and in vitro depletion of CD8 alpha+ cells. Fractionation of CD8 alpha+ T cells.
|Flow Cyt||Use 2µl for 106 cells. ab18445-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.|
|IP||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
Flow cytometry analysis showing C57Bl/6 splenocytes stained with ab25176 (solid line). Isotype control antibody was Rat Ig2a Biotin (dashed line).
ab25176 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"