Anti-CD8 alpha antibody [53-6.7] (Biotin) (ab25176)

Overview

  • Product nameAnti-CD8 alpha antibody [53-6.7] (Biotin)
    See all CD8 alpha primary antibodies
  • Description
    Rat monoclonal [53-6.7] to CD8 alpha (Biotin)
  • ConjugationBiotin
  • Specificityab25176 recognises a chain of the murine CD8 heterodimer, Mr 32-34 kDa.
  • Tested applicationsSuitable for: Functional Studies, Flow Cyt, IP, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • General notes


    In vivo and in vitro treatment with ab25176 effectively depletes CD8 alpha+ cells. ab25176 also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityIgG fraction
  • Primary antibody notesIn vivo and in vitro treatment with ab25176 effectively depletes CD8 alpha+ cells. ab25176 also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.
  • ClonalityMonoclonal
  • Clone number53-6.7
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25176 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

In vivo and in vitro depletion of CD8 alpha+ cells. Fractionation of CD8 alpha+ T cells.

Flow Cyt Use 2µl for 106 cells. ab18445-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Target

  • FunctionIdentifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
  • Involvement in diseaseDefects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
  • Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • alpha polypeptide (p32) antibody
    • CD8 antibody
    • CD8 antigen alpha polypeptide antibody
    • CD8 antigen alpha polypeptide (p32) antibody
    • CD8a antibody
    • CD8A antigen antibody
    • CD8A molecule antibody
    • CD8A_HUMAN antibody
    • Leu2 antibody
    • Leu2 T lymphocyte antigen antibody
    • Ly3 antibody
    • LYT3 antibody
    • MAL antibody
    • OKT8 T cell antigen antibody
    • OTTHUMP00000160760 antibody
    • OTTHUMP00000160764 antibody
    • OTTHUMP00000203528 antibody
    • OTTHUMP00000203721 antibody
    • p32 antibody
    • T cell antigen Leu2 antibody
    • T cell co receptor antibody
    • T-cell surface glycoprotein CD8 alpha chain antibody
    • T-lymphocyte differentiation antigen T8/Leu-2 antibody
    • T8 T cell antigen antibody
    • T8/Leu-2 T-lymphocyte differentiation antigen antibody
    see all

Anti-CD8 alpha antibody [53-6.7] (Biotin) images

References for Anti-CD8 alpha antibody [53-6.7] (Biotin) (ab25176)

ab25176 has not yet been referenced specifically in any publications.

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I have received following reply from my colleague when asked for immunogen;
"The immunogen used to produce clone 53-6.7 was mouse thymus or spleen. Generation of this clone is described in the below reference artic...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"