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The details of the immunogen for this antibody are not available.
Our Abpromise guarantee covers the use of ab25676 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 2µg for 106 cells.
ab18446 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|IHC-Fr||Use at an assay dependent concentration. Fix with acetone.|
|IP||Use at an assay dependent concentration.|
|Blocking||Use at an assay dependent concentration. Blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2|
|Functional Studies||Use at an assay dependent concentration. In vivo and in vitro depletion of CD8alpha cells; fractionation of CD8alpha T cells.|
ab25676 has not yet been referenced specifically in any publications.