The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.125µg for 105-8 cells. Staining the cell sample in a final volume of 100 µL is recommended.
FunctionIdentifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
Involvement in diseaseDefects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
Staining of BALB/c splenocytes with staining buffer (autofluorescence) (open histogram) or 0.06 µg of ab95588
(filled histogram). Total cells were used for analysis.
References for Anti-CD8 alpha antibody [53-6.7] (PE/Cy5®) (ab95588)
This product has been referenced in:
Yang Z et al. Myocardial infarct-sparing effect of adenosine A2A receptor activation is due to its action on CD4+ T lymphocytes. Circulation114:2056-64 (2006).
Read more (PubMed: 17060376) »
Taylor JL et al. Factors associated with severe granulomatous pneumonia in Mycobacterium tuberculosis-infected mice vaccinated therapeutically with hsp65 DNA. Infect Immun73:5189-93 (2005).
Read more (PubMed: 16041037) »