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The details of the immunogen for this antibody are not available.
In vivo and in vitro treatment with the 53-6.7 monoclonal antibody effectively depletes CD8 alpha+ cells. The 53-6.7 monoclonal antibody also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.
Our Abpromise guarantee covers the use of ab39853 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 0.2µg for 106 cells. ab154460-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.|
|IHC-Fr||Use at an assay dependent concentration.|
|Functional Studies||Use at an assay dependent concentration.
In vivo and in vitro depletion of CD8a+ cells and fractionation of CD8a+ T cells.
|IP||Use at an assay dependent concentration.|