The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.2µg for 106 cells. ab134674-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
Use at an assay dependent concentration. Fix with acetone.
Use at an assay dependent concentration.
Use at an assay dependent concentration. (In vivo and in vitro depletion of CD8a+ cells; Fractionation of CD8a+ T cells)
FunctionIdentifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
Involvement in diseaseDefects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
BALB/c mesenteric lymph node cells were first labeled with anti Integrin alpha E (ab25211) at 0.1 µg/106 cells, and then double-stained with streptavidin-FITC and ab25498. Small lymphocytes were gated and analyzed on a FACScan flow cytometer (BDIS, San Jose, CA).
References for Anti-CD8 alpha antibody [53-6.7] (Phycoerythrin) (ab25498)
This product has been referenced in:
Trautmann T et al. CD4+ T-cell help is required for effective CD8+ T cell-mediated resolution of acute viral hepatitis in mice. PLoS One9:e86348 (2014).
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