Anti-CD8 alpha antibody [53-6.7] (Phycoerythrin -Texas Red®) (ab25294)


  • Product nameAnti-CD8 alpha antibody [53-6.7] (Phycoerythrin -Texas Red®)
    See all CD8 alpha primary antibodies
  • Description
    Rat monoclonal [53-6.7] to CD8 alpha (Phycoerythrin -Texas Red®)
  • ConjugationPhycoerythrin -Texas Red®. Ex: 496nm, Em: 613nm
  • Specificityab25194 recognises CD8 alpha. In vivo and in vitro treatment with ab25294 effectively depletes CD8a+ cells. It also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.
  • Tested applicationsSuitable for: Flow Cyt, IHC-Fr, IPmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    The details of the immunogen for this antibody are not available.



Our Abpromise guarantee covers the use of ab25294 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.2µg for 106 cells.
IHC-Fr Use at an assay dependent dilution.
IP Use at an assay dependent dilution.
In vivo and in vitro depletion of CD8alpha+ cells: Use at an assay dependent dilution.
Fractionation of CD8alpha+ T cells: Use at an assay dependent dilution.


  • FunctionIdentifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
  • Involvement in diseaseDefects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
  • Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • alpha polypeptide (p32) antibody
    • CD8 antibody
    • CD8 antigen alpha polypeptide antibody
    • CD8 antigen alpha polypeptide (p32) antibody
    • CD8a antibody
    • CD8A antigen antibody
    • CD8A molecule antibody
    • CD8A_HUMAN antibody
    • Leu2 antibody
    • Leu2 T lymphocyte antigen antibody
    • Ly3 antibody
    • LYT3 antibody
    • MAL antibody
    • OKT8 T cell antigen antibody
    • OTTHUMP00000160760 antibody
    • OTTHUMP00000160764 antibody
    • OTTHUMP00000203528 antibody
    • OTTHUMP00000203721 antibody
    • p32 antibody
    • T cell antigen Leu2 antibody
    • T cell co receptor antibody
    • T-cell surface glycoprotein CD8 alpha chain antibody
    • T-lymphocyte differentiation antigen T8/Leu-2 antibody
    • T8 T cell antigen antibody
    • T8/Leu-2 T-lymphocyte differentiation antigen antibody
    see all

References for Anti-CD8 alpha antibody [53-6.7] (Phycoerythrin -Texas Red®) (ab25294)

This product has been referenced in:
  • Tomov VT  et al. Persistent enteric murine norovirus infection is associated with functionally suboptimal virus-specific CD8 T cell responses. J Virol 87:7015-31 (2013). Read more (PubMed: 23596300) »
  • Hall JM  et al. Chronic psychological stress suppresses contact hypersensitivity: Potential roles of dysregulated cell trafficking and decreased IFN-? production. Brain Behav Immun N/A:N/A (2013). Mouse . Read more (PubMed: 24184400) »

See all 4 Publications for this product

Product Wall

Thank you for your enquiry.

We do not have set expiration dates for our products. Most antibodies are stable and can last for anywhere from a few months to several years if stored properly, so we strongly recommend that you follow the storage ...

Read More