Overview

  • Product name
    Anti-CD8 alpha antibody [CA9.JD3]
    See all CD8 alpha primary antibodies
  • Description
    Mouse monoclonal [CA9.JD3] to CD8 alpha
  • Specificity
    Recognizes canine homologue CD8 alpha chain which is expressed by a thymocytes, peripheral T cells and a splenic zone macrophages.
  • Tested applications
    Suitable for: Flow Cyt, IHC-Fr, IPmore details
  • Species reactivity
    Reacts with: Dog
  • Immunogen

    Thymocytes (Dog)

  • Positive control
    • Spleen, lymphnode
  • General notes


    Clone CA9.JD3 has been reported to inhibit cytotoxic T lymphocyte function. Removal of sodium azide is recommended prior to use in functional assays.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Primary antibody notes
    Clone CA9.JD3 has been reported to inhibit cytotoxic T lymphocyte function. Removal of sodium azide is recommended prior to use in functional assays.
  • Clonality
    Monoclonal
  • Clone number
    CA9.JD3
  • Myeloma
    P3x63-Ag8.653
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab34105 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 10µl for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-Fr 1/1 - 1/10.
IP Use at an assay dependent concentration.

Target

  • Function
    Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
  • Involvement in disease
    Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
  • Sequence similarities
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Alternative names
    • alpha polypeptide (p32) antibody
    • CD8 antibody
    • CD8 antigen alpha polypeptide antibody
    • CD8 antigen alpha polypeptide (p32) antibody
    • CD8a antibody
    • CD8A antigen antibody
    • CD8A molecule antibody
    • CD8A_HUMAN antibody
    • Leu2 antibody
    • Leu2 T lymphocyte antigen antibody
    • Ly3 antibody
    • LYT3 antibody
    • MAL antibody
    • OKT8 T cell antigen antibody
    • OTTHUMP00000160760 antibody
    • OTTHUMP00000160764 antibody
    • OTTHUMP00000203528 antibody
    • OTTHUMP00000203721 antibody
    • p32 antibody
    • T cell antigen Leu2 antibody
    • T cell co receptor antibody
    • T-cell surface glycoprotein CD8 alpha chain antibody
    • T-lymphocyte differentiation antigen T8/Leu-2 antibody
    • T8 T cell antigen antibody
    • T8/Leu-2 T-lymphocyte differentiation antigen antibody
    see all

References

This product has been referenced in:
  • Cobbold S & Metcalfe S Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW). Tissue Antigens 43:137-54 (1994). Read more (PubMed: 8091414) »
  • Moore PF  et al. Monoclonal antibodies specific for canine CD4 and CD8 define functional T-lymphocyte subsets and high-density expression of CD4 by canine neutrophils. Tissue Antigens 40:75-85 (1992). Read more (PubMed: 1412420) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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