• Product nameAnti-CD8 alpha antibody [CT-8]
    See all CD8 alpha primary antibodies
  • Description
    Mouse monoclonal [CT-8] to CD8 alpha
  • Specificityab25346 recognizes a chain of chicken CD8.
  • Tested applicationsSuitable for: Flow Cyt, IHC-Fr, IPmore details
  • Species reactivity
    Reacts with: Chicken
  • Immunogen

    The details of the immunogen for this antibody are not available.



Our Abpromise guarantee covers the use of ab25346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.


  • FunctionIdentifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
  • Involvement in diseaseDefects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
  • Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Alternative names
    • alpha polypeptide (p32) antibody
    • CD8 antibody
    • CD8 antigen alpha polypeptide antibody
    • CD8 antigen alpha polypeptide (p32) antibody
    • CD8a antibody
    • CD8A antigen antibody
    • CD8A molecule antibody
    • CD8A_HUMAN antibody
    • Leu2 antibody
    • Leu2 T lymphocyte antigen antibody
    • Ly3 antibody
    • LYT3 antibody
    • MAL antibody
    • OKT8 T cell antigen antibody
    • OTTHUMP00000160760 antibody
    • OTTHUMP00000160764 antibody
    • OTTHUMP00000203528 antibody
    • OTTHUMP00000203721 antibody
    • p32 antibody
    • T cell antigen Leu2 antibody
    • T cell co receptor antibody
    • T-cell surface glycoprotein CD8 alpha chain antibody
    • T-lymphocyte differentiation antigen T8/Leu-2 antibody
    • T8 T cell antigen antibody
    • T8/Leu-2 T-lymphocyte differentiation antigen antibody
    see all

References for Anti-CD8 alpha antibody [CT-8] (ab25346)

This product has been referenced in:

See 1 Publication for this product

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Thank you for contacting us.

1. Yes, a substrate is needed.

2. DABor AEC can be used with HRP. I would recommend one ofthe following products :
> IHC detectionkits 'EXPOSE': http://www.abcam.com/expose (see the 6 first refer...

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1. yes,you need a secondary antibody such as ab97239 (Goat anti-Mouse IgG1 heavy chain (FITC) secondary antibody). The reason isthat the primary antibodies you want to use (ab25345, ab25346 andab25347) are not conjugated.

2. a.Yes, ab98693 (G...

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Yes,the chosen antibodies are suitable for Flow Cytometry and chicken samples.

No, none of the chosen primary antibodies (ab25345, ab25346, ab25347) are conjugated, so you need a secondary antibody such as ab97239.

The secondary ant...

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Thank you for contacting us.

I am happy to confirm that your chosen antibodies are suitable in Flow Cytometry and chicken, and that nothing else should be required.

However, I am wondering about the usage of the ab37382 Chicken IgY...

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