Anti-CD8 alpha antibody [CT-8] (Phycoerythrin) (ab25422)

Overview

  • Product nameAnti-CD8 alpha antibody [CT-8] (Phycoerythrin)
    See all CD8 alpha primary antibodies
  • Description
    Mouse monoclonal [CT-8] to CD8 alpha (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • Specificityab25422 recognises CD8 alpha.
  • Tested applicationsSuitable for: Flow Cyt, IHC-Fr, IPmore details
  • Species reactivity
    Reacts with: Chicken
  • Immunogen

    The details of the immunogen for this antibody are not available.

Properties

Applications

Our Abpromise guarantee covers the use of ab25422 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
IHC-Fr
IP
  • Application notesFlow Cyt: Use 0.2µg for 106 cells.
    IHC-Fr: Use at an assay dependent dilution.
    Note: samples need to be Acetone fixed.
    IP: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionIdentifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
    • Involvement in diseaseDefects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
    • Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Post-translational
      modifications
      All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
    • Cellular localizationSecreted and Cell membrane.
    • Information by UniProt
    • Alternative names
      • alpha polypeptide (p32) antibody
      • CD8 antibody
      • CD8 antigen alpha polypeptide antibody
      • CD8 antigen alpha polypeptide (p32) antibody
      • CD8a antibody
      • CD8A antigen antibody
      • CD8A molecule antibody
      • CD8A_HUMAN antibody
      • Leu2 antibody
      • Leu2 T lymphocyte antigen antibody
      • Ly3 antibody
      • LYT3 antibody
      • MAL antibody
      • OKT8 T cell antigen antibody
      • OTTHUMP00000160760 antibody
      • OTTHUMP00000160764 antibody
      • OTTHUMP00000203528 antibody
      • OTTHUMP00000203721 antibody
      • p32 antibody
      • T cell antigen Leu2 antibody
      • T cell co receptor antibody
      • T-cell surface glycoprotein CD8 alpha chain antibody
      • T-lymphocyte differentiation antigen T8/Leu-2 antibody
      • T8 T cell antigen antibody
      • T8/Leu-2 T-lymphocyte differentiation antigen antibody
      see all

    Anti-CD8 alpha antibody [CT-8] (Phycoerythrin) images

    • Chicken PBMC were double stained with mouse anti chicken CD4 FITC and ab25422 (0.2 µg/106 cells). Small lymphocytes were then gated and analyzed on a flow cytometer.

    References for Anti-CD8 alpha antibody [CT-8] (Phycoerythrin) (ab25422)

    ab25422 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab25422.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"