Recombinant
RabMAb

Anti-CD8 alpha antibody [EP1150] (ab108343)

Overview

  • Product name
    Anti-CD8 alpha antibody [EP1150]
    See all CD8 alpha primary antibodies
  • Description
    Rabbit monoclonal [EP1150] to CD8 alpha
  • Tested applications
    Suitable for: IP, IHC-P, ICC, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues near the C-terminus of Human CD8 alpha.

  • Positive control
    • MOLT-4, Jurkat cell lysates, Human tonsil tissue
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab108343 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/10.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
Flow Cyt 1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
  • Involvement in disease
    Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
  • Sequence similarities
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • alpha polypeptide (p32) antibody
    • CD8 antibody
    • CD8 antigen alpha polypeptide antibody
    • CD8 antigen alpha polypeptide (p32) antibody
    • CD8a antibody
    • CD8A antigen antibody
    • CD8A molecule antibody
    • CD8A_HUMAN antibody
    • Leu2 antibody
    • Leu2 T lymphocyte antigen antibody
    • Ly3 antibody
    • LYT3 antibody
    • MAL antibody
    • OKT8 T cell antigen antibody
    • OTTHUMP00000160760 antibody
    • OTTHUMP00000160764 antibody
    • OTTHUMP00000203528 antibody
    • OTTHUMP00000203721 antibody
    • p32 antibody
    • T cell antigen Leu2 antibody
    • T cell co receptor antibody
    • T-cell surface glycoprotein CD8 alpha chain antibody
    • T-lymphocyte differentiation antigen T8/Leu-2 antibody
    • T8 T cell antigen antibody
    • T8/Leu-2 T-lymphocyte differentiation antigen antibody
    see all

Images

  • Flow cytometric analysis of paraformaldehyde-fixed human peripheral blood mononuclear cells (PBMC) labeling CD8 alpha with ab108343 at 1/40 dilution, followed by F(ab')2 Frag Goat Anti-Rabbit IgG (H+L) Alexa Fluor® 488.

    Gating Strategy: lymphocytes based on FSC vs SSC.

    See Abreview

  • ab108343 at 1/250 dilution staining CD8 alpha in Human tonsil tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.

References

This product has been referenced in:
  • Mori K  et al. Systemic Analysis of Predictive Biomarkers for Recurrence in Colorectal Cancer Patients Treated with Curative Surgery. Dig Dis Sci 60:2477-87 (2015). IHC ; Human . Read more (PubMed: 25840921) »
  • Qin L  et al. CD4+ and CD8+ T lymphocytes in lung tissue of NSIP: correlation with T lymphocytes in BALF. Respir Med 107:120-7 (2013). IHC-P ; Human . Read more (PubMed: 23085212) »

See all 2 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (peripheral blood mononuclear cells (PBMC))
Permeabilization
Yes - 0.5% saponin
Gating Strategy
lymphocytes based on FSC vs SSC
Specification
peripheral blood mononuclear cells (PBMC)
Fixation
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 13 2016

Thank you very much for your phone call.

The best antibodies we can recommend are as follows. Please note that the recommendations are based on immunogen sequence only we currently do not hold any rabbit specific data.

Anti CD3 : ab...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Colon)
Specification
Colon
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Blocking step
(agent) for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Dr. R Berahovich

Verified customer

Submitted Dec 16 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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