The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.5µg for 106 cells. ab91356-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionIdentifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
Involvement in diseaseDefects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
ab111919 at 0.5 µg/106 cells detecting CD8 alpha in Fischer Rat thymus cells by Flow Cytometry. 95% more of the cells were stained than in the control (FITC Mouse IgG1, k). Cells from Rat strains Wistar, Buffalo and Brown Norway were also positive in this assay.
References for Anti-CD8 alpha antibody [MRC OX-8] (FITC) (ab111919)
This product has been referenced in:
Johnson P et al. Purification, chain separation and sequence of the MRC OX-8 antigen, a marker of rat cytotoxic T lymphocytes. EMBO J4:2539-45 (1985).
Read more (PubMed: 3932064) »
Thomas ML & Green JR Molecular nature of the W3/25 and MRC OX-8 marker antigens for rat T lymphocytes: comparisons with mouse and human antigens. Eur J Immunol13:855-8 (1983).
Read more (PubMed: 6416869) »