Overview

  • Product nameAnti-CD8 alpha antibody [OX-8]
    See all CD8 alpha primary antibodies
  • Description
    Mouse monoclonal [OX-8] to CD8 alpha
  • Tested applicationsSuitable for: Blocking, Flow Cyt, IHC-Fr, IHC-P, WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Rat thymocyte membrane glycoproteins.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone numberOX-8
  • MyelomaNS1
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab33786 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Blocking Use at an assay dependent concentration.
Flow Cyt 1/50 - 1/100. Use 10µl of the suggested working dilution to label 106 cells in 100µl.



ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionIdentifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
  • Involvement in diseaseDefects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
  • Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • alpha polypeptide (p32) antibody
    • CD8 antibody
    • CD8 antigen alpha polypeptide antibody
    • CD8 antigen alpha polypeptide (p32) antibody
    • CD8a antibody
    • CD8A antigen antibody
    • CD8A molecule antibody
    • CD8A_HUMAN antibody
    • Leu2 antibody
    • Leu2 T lymphocyte antigen antibody
    • Ly3 antibody
    • LYT3 antibody
    • MAL antibody
    • OKT8 T cell antigen antibody
    • OTTHUMP00000160760 antibody
    • OTTHUMP00000160764 antibody
    • OTTHUMP00000203528 antibody
    • OTTHUMP00000203721 antibody
    • p32 antibody
    • T cell antigen Leu2 antibody
    • T cell co receptor antibody
    • T-cell surface glycoprotein CD8 alpha chain antibody
    • T-lymphocyte differentiation antigen T8/Leu-2 antibody
    • T8 T cell antigen antibody
    • T8/Leu-2 T-lymphocyte differentiation antigen antibody
    see all

Anti-CD8 alpha antibody [OX-8] images

  • ab33786 staining CD8 alpha in rat spleen tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed with 10% neutral buffered formalin and a heat mediated antigen retrieval step was performed using citrate buffer. Samples were then permeabilized with Triton X-100, blocked using 5% serum for 30 minutes at 25°C and then incubated with ab33786 at a 1/100 dilution for 1 hour at 25°C. The secondary used was a biotin-conjugated rabbit anti-mouse polyclonal, used at a 1/300 dilution.

    See Abreview

  • ab33786 staining CD8 alpha in rat spleen tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed in acetone, blocked with 5% serum for 30 minutes at 25°C, then incubated with ab33786 at a 1/200 for 1 hour 30 minutes at 25°C. The secondary used was a biotin conjugated rabbit anti-mouse polyclonal, used at a 1/300.

    See Abreview

References for Anti-CD8 alpha antibody [OX-8] (ab33786)

This product has been referenced in:
  • Muhamed J  et al. Comparative local immunogenic potential of scaffolds prepared from porcine cholecyst, jejunum, and urinary bladder in rat subcutaneous model. J Biomed Mater Res B Appl Biomater N/A:N/A (2014). Read more (PubMed: 25370716) »
  • Whiteland JL  et al. Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies. J Histochem Cytochem 43:313-20 (1995). Read more (PubMed: 7868861) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (spleen)
Specification spleen
Fixative Acetone
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 07 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (spleen)
Specification spleen
Fixative 10 % Neutral Buffered Formalin
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization Yes - Triton X-100
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 06 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"