Overview

  • Product nameAnti-CD8 alpha antibody [OX-8]
    See all CD8 alpha primary antibodies
  • Description
    Mouse monoclonal [OX-8] to CD8 alpha
  • Tested applicationsSuitable for: Blocking, Flow Cyt, IHC-Fr, IHC-P, Functional Studiesmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Rat thymocyte membrane glycoproteins.

  • Positive control
    • Rat splenocytes.

Properties

Applications

Our Abpromise guarantee covers the use of ab33787 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Blocking Use at an assay dependent concentration.
Flow Cyt 1/100 - 1/200. Use 10µl of the suggested working dilution to label 106 cells in 100µl.



ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration.

Target

  • FunctionIdentifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
  • Involvement in diseaseDefects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
  • Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • alpha polypeptide (p32) antibody
    • CD8 antibody
    • CD8 antigen alpha polypeptide antibody
    • CD8 antigen alpha polypeptide (p32) antibody
    • CD8a antibody
    • CD8A antigen antibody
    • CD8A molecule antibody
    • CD8A_HUMAN antibody
    • Leu2 antibody
    • Leu2 T lymphocyte antigen antibody
    • Ly3 antibody
    • LYT3 antibody
    • MAL antibody
    • OKT8 T cell antigen antibody
    • OTTHUMP00000160760 antibody
    • OTTHUMP00000160764 antibody
    • OTTHUMP00000203528 antibody
    • OTTHUMP00000203721 antibody
    • p32 antibody
    • T cell antigen Leu2 antibody
    • T cell co receptor antibody
    • T-cell surface glycoprotein CD8 alpha chain antibody
    • T-lymphocyte differentiation antigen T8/Leu-2 antibody
    • T8 T cell antigen antibody
    • T8/Leu-2 T-lymphocyte differentiation antigen antibody
    see all

References for Anti-CD8 alpha antibody [OX-8] (ab33787)

This product has been referenced in:
  • Popov I  et al. The effect of an anti-HLA-B27 immune response on CTL recognition of Chlamydia. J Immunol 167:3375-82 (2001). Read more (PubMed: 11544328) »
  • Whiteland JL  et al. Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies. J Histochem Cytochem 43:313-20 (1995). Read more (PubMed: 7868861) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"