Anti-CD8 alpha antibody [RPA-T8] (Allophycocyanin) (ab86892)

Overview

  • Product nameAnti-CD8 alpha antibody [RPA-T8] (Allophycocyanin)
    See all CD8 alpha primary antibodies
  • Description
    Mouse monoclonal [RPA-T8] to CD8 alpha (Allophycocyanin)
  • ConjugationAllophycocyanin. Ex: 645nm, Em: 660nm
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human, Monkey
  • Immunogen

    CD8 (CD8a; alpha chain)

  • Positive control
    • Human PBMCs.

Properties

Applications

Our Abpromise guarantee covers the use of ab86892 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notesFlow Cyt: Use 10µl (or 0.1µg) for 106 cells.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionIdentifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
    • Involvement in diseaseDefects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
    • Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Post-translational
      modifications
      All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
    • Cellular localizationSecreted and Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • alpha polypeptide (p32) antibody
      • CD8 antibody
      • CD8 antigen alpha polypeptide antibody
      • CD8 antigen alpha polypeptide (p32) antibody
      • CD8a antibody
      • CD8A antigen antibody
      • CD8A molecule antibody
      • CD8A_HUMAN antibody
      • Leu2 antibody
      • Leu2 T lymphocyte antigen antibody
      • Ly3 antibody
      • LYT3 antibody
      • MAL antibody
      • OKT8 T cell antigen antibody
      • OTTHUMP00000160760 antibody
      • OTTHUMP00000160764 antibody
      • OTTHUMP00000203528 antibody
      • OTTHUMP00000203721 antibody
      • p32 antibody
      • T cell antigen Leu2 antibody
      • T cell co receptor antibody
      • T-cell surface glycoprotein CD8 alpha chain antibody
      • T-lymphocyte differentiation antigen T8/Leu-2 antibody
      • T8 T cell antigen antibody
      • T8/Leu-2 T-lymphocyte differentiation antigen antibody
      see all

    Anti-CD8 alpha antibody [RPA-T8] (Allophycocyanin) images

    • Flow Cytometry of CD8 in human PBMCs using 10 µl of ab86892. Shaded histogram represents cells without antibody; green represents isotype control; purple represents anti-CD8 antibody.

    References for Anti-CD8 alpha antibody [RPA-T8] (Allophycocyanin) (ab86892)

    ab86892 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for submitting the Abreviews for ab86892. As you may have noticed, your reviews have now been published on our website.
    Since you obtained poor results using the antibody in untested species (mouse and rat), we would like to follow up on ...

    Read More
    Abcam has not validated the combination of species/application used in this Abreview.
    Application Flow Cytometry
    Sample Mouse Cell (Splenocytes)
    Specification Splenocytes
    Preparation Cell harvesting/tissue preparation method: harvesting of spleen
    Sample buffer: PBS/BSA 1%
    Permeabilization No
    Gating Strategy alive cells
    Username

    Abcam user community

    Verified customer

    Submitted Nov 14 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Flow Cytometry
    Sample Monkey Cell (leucocytes from whole blood)
    Specification leucocytes from whole blood
    Preparation Cell harvesting/tissue preparation method: after red blood cell lysis
    Sample buffer: PBS/BSA 1%
    Fixation Paraformaldehyde
    Permeabilization No
    Gating Strategy alive cells
    Username

    Abcam user community

    Verified customer

    Submitted Nov 14 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Flow Cytometry
    Sample Human Cell (leucocytes from whole blood)
    Specification leucocytes from whole blood
    Preparation Cell harvesting/tissue preparation method: after red blood cell lysis
    Sample buffer: PBS/BSA 1%
    Fixation Paraformaldehyde
    Permeabilization No
    Gating Strategy alive lymphocytes
    Username

    Abcam user community

    Verified customer

    Submitted Nov 14 2012

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Flow Cytometry
    Sample Rat Cell (splenocytes)
    Specification splenocytes
    Preparation Cell harvesting/tissue preparation method: harvesting of spleen
    Sample buffer: PBS/BSA 1%
    Permeabilization No
    Gating Strategy alive cells
    Username

    Abcam user community

    Verified customer

    Submitted Nov 14 2012

    Thank you for submitting an Abreview for ab86892. As you may have noticed, your review has now been published on our website.
    Since you obtained poor results using the antibody in an untested species (dog), we would like to follow up on this to see...

    Read More
    Abcam has not validated the combination of species/application used in this Abreview.
    Application Flow Cytometry
    Sample Dog Cell (from whole blood)
    Specification from whole blood
    Preparation Cell harvesting/tissue preparation method: after red blood cell lysis
    Sample buffer: PBS/BSA 1%
    Fixation Paraformaldehyde
    Permeabilization No
    Gating Strategy alive lymphocytes
    Username

    Abcam user community

    Verified customer

    Submitted Nov 07 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"