The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 5µl for 106 cells. ab67435-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
Involvement in disease
Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
Staining of normal Human peripheral blood cells with Mouse IgG1 isotype control PE-Cy5 (open histogram) or ab95595
(filled histogram). Cells in the lymphocyte gate were used for analysis.
Flow Cytometry - Anti-CD8 alpha antibody [RPA-T8] (PE/Cy5®) (ab95595)This image is courtesy of an anonymous Abreview
ab95595 staining CD8 alpha in Human PBMCs cell by Flow Cytometry. Cells were harvested by Ficoll-hypaque gradient. The sample was incubated with the primary antibody (1/100 2% Human serum, 1mM EDTA in PBS) for 25 minutes at 4°C.
Gating Strategy: Lymphocytes.