Anti-CD8 alpha antibody [RPA-T8] (PE/Cy5®) (ab95595)

Overview

  • Product nameAnti-CD8 alpha antibody [RPA-T8] (PE/Cy5®)
    See all CD8 alpha primary antibodies
  • Description
    Mouse monoclonal [RPA-T8] to CD8 alpha (PE/Cy5®)
  • ConjugationPE/Cy5®. Ex: 496nm, Em: 670nm
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    hPBMC stimulated with PHA.

  • Positive control
    • Normal Human peripheral blood cells

Properties

Applications

Our Abpromise guarantee covers the use of ab95595 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 5µl for 106 cells. ab67435-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionIdentifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
  • Involvement in diseaseDefects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
  • Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • alpha polypeptide (p32) antibody
    • CD8 antibody
    • CD8 antigen alpha polypeptide antibody
    • CD8 antigen alpha polypeptide (p32) antibody
    • CD8a antibody
    • CD8A antigen antibody
    • CD8A molecule antibody
    • CD8A_HUMAN antibody
    • Leu2 antibody
    • Leu2 T lymphocyte antigen antibody
    • Ly3 antibody
    • LYT3 antibody
    • MAL antibody
    • OKT8 T cell antigen antibody
    • OTTHUMP00000160760 antibody
    • OTTHUMP00000160764 antibody
    • OTTHUMP00000203528 antibody
    • OTTHUMP00000203721 antibody
    • p32 antibody
    • T cell antigen Leu2 antibody
    • T cell co receptor antibody
    • T-cell surface glycoprotein CD8 alpha chain antibody
    • T-lymphocyte differentiation antigen T8/Leu-2 antibody
    • T8 T cell antigen antibody
    • T8/Leu-2 T-lymphocyte differentiation antigen antibody
    see all

Anti-CD8 alpha antibody [RPA-T8] (PE/Cy5®) images

  • Staining of normal Human peripheral blood cells with Mouse IgG1 isotype control PE-Cy5 (open histogram) or ab95595 (filled histogram). Cells in the lymphocyte gate were used for analysis.
  • ab95595 staining CD8 alpha in Human PBMCs cell by Flow Cytometry. Cells were harvested by Ficoll-hypaque gradient. The sample was incubated with the primary antibody (1/100 2% Human serum, 1mM EDTA in PBS) for 25 minutes at 4°C.
    Gating Strategy: Lymphocytes.

    See Abreview

References for Anti-CD8 alpha antibody [RPA-T8] (PE/Cy5®) (ab95595)

ab95595 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (PBMC)
Specification PBMC
Preparation Cell harvesting/tissue preparation method: Ficoll-hypaque gradrient
Sample buffer: PBS
Permeabilization No
Gating Strategy lymphocytes
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Submitted Feb 19 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"