Anti-CD8 alpha antibody [YTS 169AG 101HL] (FITC) (ab112219)

Overview

  • Product nameAnti-CD8 alpha antibody [YTS 169AG 101HL] (FITC)
    See all CD8 alpha primary antibodies
  • Description
    Rat monoclonal [YTS 169AG 101HL] to CD8 alpha (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Specificityab112219 reacts with CD8alpha found on Mouse thymocytes and Mouse cytotoxic/suppressor T cells. It does not bind to Mouse helper/inducer T cells. It binds to T lymphocytes from all Mouse strains regardless of phenotypic expression (i.e. reacts with T lymphocytes from mouse strains expressing the Ly 2.1 or Ly 2.2 phenotype).
  • Tested applicationsSuitable for: IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue, cells or virus corresponding to Mouse CD8 alpha. Mouse CD8alpha thymocytes

  • Positive control
    • Mouse Strain: ATH (Thymus or Splenic T -cells)

Properties

Applications

Our Abpromise guarantee covers the use of ab112219 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Antigen retrieval is required
Flow Cyt Use 0.1µg for 106 cells. ab136125-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Target

  • FunctionIdentifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
  • Involvement in diseaseDefects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
  • Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • alpha polypeptide (p32) antibody
    • CD8 antibody
    • CD8 antigen alpha polypeptide antibody
    • CD8 antigen alpha polypeptide (p32) antibody
    • CD8a antibody
    • CD8A antigen antibody
    • CD8A molecule antibody
    • CD8A_HUMAN antibody
    • Leu2 antibody
    • Leu2 T lymphocyte antigen antibody
    • Ly3 antibody
    • LYT3 antibody
    • MAL antibody
    • OKT8 T cell antigen antibody
    • OTTHUMP00000160760 antibody
    • OTTHUMP00000160764 antibody
    • OTTHUMP00000203528 antibody
    • OTTHUMP00000203721 antibody
    • p32 antibody
    • T cell antigen Leu2 antibody
    • T cell co receptor antibody
    • T-cell surface glycoprotein CD8 alpha chain antibody
    • T-lymphocyte differentiation antigen T8/Leu-2 antibody
    • T8 T cell antigen antibody
    • T8/Leu-2 T-lymphocyte differentiation antigen antibody
    see all

Anti-CD8 alpha antibody [YTS 169AG 101HL] (FITC) images

  • Flow Cytometry staining of CD8alpha using ab112219 at 0.1 µg/106 cells.

    Mouse Strain: ATH, Cell Source: Thymus.

References for Anti-CD8 alpha antibody [YTS 169AG 101HL] (FITC) (ab112219)

This product has been referenced in:
  • Handisurya A  et al. Strain-specific properties and T cells regulate the susceptibility to papilloma induction by Mus musculus papillomavirus 1. PLoS Pathog 10:e1004314 (2014). Flow Cyt ; Mouse . Read more (PubMed: 25121947) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"