Rat monoclonal [YTS 169AG 101HL] to CD8 alpha (FITC)
FITC. Ex: 493nm, Em: 528nm
ab112219 reacts with CD8alpha found on Mouse thymocytes and Mouse cytotoxic/suppressor T cells. It does not bind to Mouse helper/inducer T cells. It binds to T lymphocytes from all Mouse strains regardless of phenotypic expression (i.e. reacts with T lymphocytes from mouse strains expressing the Ly 2.1 or Ly 2.2 phenotype).
Tissue, cells or virus corresponding to Mouse CD8 alpha. Mouse CD8alpha thymocytes
Mouse Strain: ATH (Thymus or Splenic T -cells)
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab22504 as a replacement.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. Antigen retrieval is required
Use 0.1µg for 106 cells. ab136125-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
Involvement in disease
Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
Handisurya A et al. Strain-specific properties and T cells regulate the susceptibility to papilloma induction by Mus musculus papillomavirus 1. PLoS Pathog10:e1004314 (2014).
Read more (PubMed: 25121947) »