Anti-CD8 alpha antibody [YTS 169AG 101HL] (FITC) (ab112219)

Overview

  • Product name
    Anti-CD8 alpha antibody [YTS 169AG 101HL] (FITC)
    See all CD8 alpha primary antibodies
  • Description
    Rat monoclonal [YTS 169AG 101HL] to CD8 alpha (FITC)
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Specificity
    ab112219 reacts with CD8alpha found on Mouse thymocytes and Mouse cytotoxic/suppressor T cells. It does not bind to Mouse helper/inducer T cells. It binds to T lymphocytes from all Mouse strains regardless of phenotypic expression (i.e. reacts with T lymphocytes from mouse strains expressing the Ly 2.1 or Ly 2.2 phenotype).
  • Tested applications
    Suitable for: IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue, cells or virus corresponding to Mouse CD8 alpha. Mouse CD8alpha thymocytes

  • Positive control
    • Mouse Strain: ATH (Thymus or Splenic T -cells)
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab22504 as a replacement.

Properties

Applications

Our Abpromise guarantee covers the use of ab112219 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Antigen retrieval is required
Flow Cyt Use 0.1µg for 106 cells. ab136125-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
  • Involvement in disease
    Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
  • Sequence similarities
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • alpha polypeptide (p32) antibody
    • CD8 antibody
    • CD8 antigen alpha polypeptide antibody
    • CD8 antigen alpha polypeptide (p32) antibody
    • CD8a antibody
    • CD8A antigen antibody
    • CD8A molecule antibody
    • CD8A_HUMAN antibody
    • Leu2 antibody
    • Leu2 T lymphocyte antigen antibody
    • Ly3 antibody
    • LYT3 antibody
    • MAL antibody
    • OKT8 T cell antigen antibody
    • OTTHUMP00000160760 antibody
    • OTTHUMP00000160764 antibody
    • OTTHUMP00000203528 antibody
    • OTTHUMP00000203721 antibody
    • p32 antibody
    • T cell antigen Leu2 antibody
    • T cell co receptor antibody
    • T-cell surface glycoprotein CD8 alpha chain antibody
    • T-lymphocyte differentiation antigen T8/Leu-2 antibody
    • T8 T cell antigen antibody
    • T8/Leu-2 T-lymphocyte differentiation antigen antibody
    see all

Images

  • Flow Cytometry staining of CD8alpha using ab112219 at 0.1 µg/106 cells.

    Mouse Strain: ATH, Cell Source: Thymus.

References

This product has been referenced in:
  • Handisurya A  et al. Strain-specific properties and T cells regulate the susceptibility to papilloma induction by Mus musculus papillomavirus 1. PLoS Pathog 10:e1004314 (2014). Flow Cyt ; Mouse . Read more (PubMed: 25121947) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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