Anti-CD8 alpha antibody [YTS 169AG 101HL] (Phycoerythrin) (ab112203)

Overview

  • Product nameAnti-CD8 alpha antibody [YTS 169AG 101HL] (Phycoerythrin)
    See all CD8 alpha primary antibodies
  • Description
    Rat monoclonal [YTS 169AG 101HL] to CD8 alpha (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Mouse CD8alpha thymocytes

  • Positive control
    • C57BL/6, BALB/c, AKR/J, C3H/He strains
  • General notes


    This clone has been reported to work in immunohistochemistry, frozen sections (see reference section).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.02% Sodium azide
    Constituents: 99% PBS, BSA
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesPurified from ascitic fluid via Protein G Chromatography.
  • Primary antibody notesThis clone has been reported to work in immunohistochemistry, frozen sections (see reference section).
  • ClonalityMonoclonal
  • Clone numberYTS 169AG 101HL
  • MyelomaY3/Ag1.2.3
  • IsotypeIgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab112203 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.2µg for 106 cells.

Target

  • FunctionIdentifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
  • Involvement in diseaseDefects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
  • Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • alpha polypeptide (p32) antibody
    • CD8 antibody
    • CD8 antigen alpha polypeptide antibody
    • CD8 antigen alpha polypeptide (p32) antibody
    • CD8a antibody
    • CD8A antigen antibody
    • CD8A molecule antibody
    • CD8A_HUMAN antibody
    • Leu2 antibody
    • Leu2 T lymphocyte antigen antibody
    • Ly3 antibody
    • LYT3 antibody
    • MAL antibody
    • OKT8 T cell antigen antibody
    • OTTHUMP00000160760 antibody
    • OTTHUMP00000160764 antibody
    • OTTHUMP00000203528 antibody
    • OTTHUMP00000203721 antibody
    • p32 antibody
    • T cell antigen Leu2 antibody
    • T cell co receptor antibody
    • T-cell surface glycoprotein CD8 alpha chain antibody
    • T-lymphocyte differentiation antigen T8/Leu-2 antibody
    • T8 T cell antigen antibody
    • T8/Leu-2 T-lymphocyte differentiation antigen antibody
    see all

Anti-CD8 alpha antibody [YTS 169AG 101HL] (Phycoerythrin) images

  • ?Tissue Distribution by Flow Cytometry Analysis
    Mouse Strain: BALB/c
    Cell concentration: 1x106 cells per tests
    ab112203 concentration used: 0.2 µg/106 cells
    Isotypic Control: Purified Rat IgG2b,?
    Cell source: thymus
    Percentage of cells stained above control: 87.1%

References for Anti-CD8 alpha antibody [YTS 169AG 101HL] (Phycoerythrin) (ab112203)

This product has been referenced in:
  • Stevenson PG  et al. Virus dissemination through the brain parenchyma without immunologic control. J Immunol 159:1876-84 (1997). IHC-Fr . Read more (PubMed: 9257852) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"