• Product nameAnti-CD8 antibody [341] (FITC)
    See all CD8 primary antibodies
  • Description
    Mouse monoclonal [341] to CD8 (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Rat/mouse T cell hyrids expressing CD9

  • Positive control
    • Rat lymphocytes
  • General notes

    Excitation: 488 nm Emission: 518 nm.



Our Abpromise guarantee covers the use of ab95527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.5µg for 105 cells. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number can range from 105 cells/test to 108 cells/test.

ab106163-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • FunctionIdentifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
  • Involvement in diseaseDefects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
  • Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    All of the five most C-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • FormCD8 beta tissue specificity: Isoform 1, isoform 3, isoform 5, isoform 6, isoform 7 and isoform 8 are expressed in both thymus and peripheral CD8+ T-cells. Expression of isoform 1 is higher in thymus CD8+ T-cells than in peripheral CD8+ T-cells. Expression of isoform 6 is higher in peripheral CD8+ T-cells than in thymus CD8+ T-cells. CD8 beta PTM: Phosphorylated as a consequence of T-cell activation.
  • Alternative names
    • CD8 antibody
    • CD8 antigen, alpha polypeptide (p32) antibody
    • CD8a antibody
    • CD8a antigen antibody
    • CD8a molecule antibody
    • CD8A_HUMAN antibody
    • Leu2 antibody
    • Leu2 T lymphocyte antigen antibody
    • MAL antibody
    • OKT8 T cell antigen antibody
    • p32 antibody
    • T cell antigen Leu2 antibody
    • T cell co receptor antibody
    • T-cell surface glycoprotein CD8 alpha chain antibody
    • T-lymphocyte differentiation antigen T8/Leu-2 antibody
    • T8 T cell antigen antibody
    see all

Anti-CD8 antibody [341] (FITC) images

  • Staining of rat thymocytes with 0.25 µg of Mouse IgG1 ? Isotype Control FITC (open histogram) or 0.25 µg of ab95527 FITC (filled histogram). Cells in the lymphocyte gate were used for analysis.

References for Anti-CD8 antibody [341] (FITC) (ab95527)

ab95527 has not yet been referenced specifically in any publications.

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