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Synthetic peptide comprising the 13 C-terminal amino acids of the cytoplasmic domain of CD8 alpha chain.
Our Abpromise guarantee covers the use of ab4055 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).|
|IHC-Fr||Use at an assay dependent concentration.|
ab4055 staining CD8 in Human colon tumour tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 in PBS and blocked with 5% serum for 1 hour at 24°C. Samples were incubated with primary antibody (1/500 in PBS + 0.3% Triton X-100 + 1% BSA) for 3 hours at 24°C. An undiluted Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody. Left - patient with low CD8, Right - patient with high CD8.
This image is courtesy of an anonymous Abreview
ab4055 at 1/200 staining CD8 in human tonsil tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).