Overview

  • Product nameAnti-CD8 antibody
    See all CD8 primary antibodies
  • Description
    Rabbit polyclonal to CD8
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Dog, Human, Rhesus monkey
  • Immunogen

    Synthetic peptide comprising the 13 C-terminal amino acids of the cytoplasmic domain of CD8 alpha chain.

  • Positive control
    • Tonsil.

Properties

Applications

Our Abpromise guarantee covers the use of ab4055 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
WB Use at an assay dependent concentration. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
IHC-P 1/200.
IHC-Fr Use at an assay dependent concentration.

Target

  • FunctionIdentifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
  • Involvement in diseaseDefects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
  • Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    All of the five most C-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • FormCD8 beta tissue specificity: Isoform 1, isoform 3, isoform 5, isoform 6, isoform 7 and isoform 8 are expressed in both thymus and peripheral CD8+ T-cells. Expression of isoform 1 is higher in thymus CD8+ T-cells than in peripheral CD8+ T-cells. Expression of isoform 6 is higher in peripheral CD8+ T-cells than in thymus CD8+ T-cells. CD8 beta PTM: Phosphorylated as a consequence of T-cell activation.
  • Alternative names
    • CD8 antibody
    • CD8 antigen, alpha polypeptide (p32) antibody
    • CD8a antibody
    • CD8a antigen antibody
    • CD8a molecule antibody
    • CD8A_HUMAN antibody
    • Leu2 antibody
    • Leu2 T lymphocyte antigen antibody
    • MAL antibody
    • OKT8 T cell antigen antibody
    • p32 antibody
    • T cell antigen Leu2 antibody
    • T cell co receptor antibody
    • T-cell surface glycoprotein CD8 alpha chain antibody
    • T-lymphocyte differentiation antigen T8/Leu-2 antibody
    • T8 T cell antigen antibody
    see all

Anti-CD8 antibody images

  • ab4055 staining CD8 in Human colon tumour tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 in PBS and blocked with 5% serum for 1 hour at 24°C. Samples were incubated with primary antibody (1/500 in PBS + 0.3% Triton X-100 + 1% BSA) for 3 hours at 24°C. An undiluted Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody. Left - patient with low CD8, Right - patient with high CD8.

    See Abreview

  • ab4055 staining CD8 in Human lymph node tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked for 5 minutes at 25°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/200) for 1 hour at 25°C. A Biotin-conjugated Donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • Immunofluorescence analysis of free-floating porcine brain tissue sections, staining CD8 with ab4055.

    Tissue was fixed in formalin before incubation with primary antibody (1/100). A FITC-conjugated donkey anti-rabbit IgG (1/100) was used as the secondary antibody.
  • Anti-CD8 antibody (ab4055) at 2 µg/ml + Dog spleen whole tissue lysate at 30 µg

    Secondary
    HRP-conjugated donkey anti-rabbit IgG polyclonal at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 26 kDa
    Observed band size : 26 kDa


    Exposure time : 30 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

  • ab4055 at 1/200 staining CD8 in human tonsil tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).

  • ab4055 at 1/500 dilution staining CD8 from PBMC tissue sections by IHC-P. The tissue was paraformaldehyde fixed and a heat mediated antigen retrieval step was performed prior to incubation with the antibody for 20 minutes. A biotinylated goat anti-rabbit antibody was used as the secondary.

    See Abreview

References for Anti-CD8 antibody (ab4055)

This product has been referenced in:
  • Xiao LI  et al. Multiple myeloma-associated skin light chain amyloidosis: A case of misdiagnosis. Oncol Lett 11:3617-3620 (2016). IHC ; Human . Read more (PubMed: 27284363) »
  • Wang S  et al. Interleukin-10 deficiency impairs regulatory T cell-derived neuropilin-1 functions and promotes Th1 and Th17 immunity. Sci Rep 6:24249 (2016). IHC (PFA fixed) . Read more (PubMed: 27075020) »

See all 15 Publications for this product

Product Wall

I'm very sorry to hear you are having problems with ab833 and ab4055. I would like to suggest using triton (0.3%v/v) in the buffers used to dilute the primary and secondary antibodies and an overnight incubation. A 10min high temperature antigen unm...

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step 2.5% Normal Horse Serum - VectorLabs ImmPRESS™ Excel Amplified HRP Polymer Staining Kit (Anti-Mouse Ig) as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA pH 9.0 Bond Epitope Retrieval Solution 2 (ER2, Leica)
Sample Rhesus monkey Tissue sections (Spleen)
Specification Spleen
Permeabilization No
Fixative 10% Neutral Buffered Formalin
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Abcam user community

Verified customer

Submitted Mar 26 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Diva Decloaker
Sample Human Tissue sections (Intestine)
Specification Intestine
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 23 2014

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (4-20% gradient)
Sample Dog Tissue lysate - whole (Spleen)
Specification Spleen
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Submitted Sep 26 2014

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 23°C
Sample Dog Tissue sections (Vastus Lateralis, Skeletal Muscle)
Specification Vastus Lateralis, Skeletal Muscle
Permeabilization Yes - 0.1% Triton in PBS
Fixative OCT
Username

Abcam user community

Verified customer

Submitted Sep 08 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Sample Human Tissue sections (Colon tumor)
Specification Colon tumor
Permeabilization Yes - PBS+0.3% Triton-X-100
Fixative Paraformaldehyde
Username

Abcam user community

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Submitted Aug 13 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (lymph node)
Specification lymph node
Fixative Formaldehyde
Antigen retrieval step Heat mediated
Permeabilization No
Blocking step Dako blocking reagent as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Dr. R Berahovich

Verified customer

Submitted Mar 11 2013


For ab846, it is stated on the datasheet that antigen retrieval should be done at a high pH so pH9 would be suitable.

For ab4055 it is not specified what pH should be used so I would recommend heat mediated antigen retrieval either using...

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Thank you very much for your call today.

Sinceab4055 has not been tested with mouse samples, I can offer a discount off a future purchase if you buy ab4055 now, test it with mouse samples and submit feedback to us in the form of an Abreview. ...

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rhesus monkey Tissue sections (brain)
Specification brain
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: DAKO antigen retrieval buffer , pressure cooked for 24 minutes
Permeabilization No
Blocking step (agent) for 1 hour(s) and 30 minute(s) · Concentration: 10% · Temperature: RT°C
Username

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Verified customer

Submitted Aug 20 2012

1-10 of 32 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"