Overview

  • Product name
  • Description
    Rabbit polyclonal to CD8
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Dog, Human, Rhesus monkey
  • Immunogen

    Synthetic peptide comprising the 13 C-terminal amino acids of the cytoplasmic domain of CD8 alpha chain.

  • Positive control
    • Tonsil.

Properties

Applications

Our Abpromise guarantee covers the use of ab4055 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
WB Use at an assay dependent concentration. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
IHC-P 1/200.
IHC-Fr Use at an assay dependent concentration.

Target

  • Function
    Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
  • Involvement in disease
    Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
  • Sequence similarities
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    All of the five most C-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Form
    CD8 beta tissue specificity: Isoform 1, isoform 3, isoform 5, isoform 6, isoform 7 and isoform 8 are expressed in both thymus and peripheral CD8+ T-cells. Expression of isoform 1 is higher in thymus CD8+ T-cells than in peripheral CD8+ T-cells. Expression of isoform 6 is higher in peripheral CD8+ T-cells than in thymus CD8+ T-cells. CD8 beta PTM: Phosphorylated as a consequence of T-cell activation.
  • Alternative names
    • CD8 antibody
    • CD8 antigen, alpha polypeptide (p32) antibody
    • CD8a antibody
    • CD8a antigen antibody
    • CD8a molecule antibody
    • CD8A_HUMAN antibody
    • Leu2 antibody
    • Leu2 T lymphocyte antigen antibody
    • MAL antibody
    • OKT8 T cell antigen antibody
    • p32 antibody
    • T cell antigen Leu2 antibody
    • T cell co receptor antibody
    • T-cell surface glycoprotein CD8 alpha chain antibody
    • T-lymphocyte differentiation antigen T8/Leu-2 antibody
    • T8 T cell antigen antibody
    see all

Anti-CD8 antibody images

  • ab4055 staining CD8 in human spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with the primary antibody (1/100 in TBS/BSA/azide) for 16 hours at 21°C. A biotin-conjugated goat anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody.

    See Abreview

  • ab4055 staining CD8 in Human colon tumour tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 in PBS and blocked with 5% serum for 1 hour at 24°C. Samples were incubated with primary antibody (1/500 in PBS + 0.3% Triton X-100 + 1% BSA) for 3 hours at 24°C. An undiluted Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody. Left - patient with low CD8, Right - patient with high CD8.

    See Abreview

  • ab4055 staining CD8 in Human lymph node tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked for 5 minutes at 25°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/200) for 1 hour at 25°C. A Biotin-conjugated Donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • Immunofluorescence analysis of free-floating porcine brain tissue sections, staining CD8 with ab4055.

    Tissue was fixed in formalin before incubation with primary antibody (1/100). A FITC-conjugated donkey anti-rabbit IgG (1/100) was used as the secondary antibody.
  • Anti-CD8 antibody (ab4055) at 2 µg/ml + Dog spleen whole tissue lysate at 30 µg

    Secondary
    HRP-conjugated donkey anti-rabbit IgG polyclonal at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 26 kDa
    Observed band size : 26 kDa


    Exposure time : 30 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

  • ab4055 at 1/200 staining CD8 in human tonsil tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).

  • ab4055 at 1/500 dilution staining CD8 from PBMC tissue sections by IHC-P. The tissue was paraformaldehyde fixed and a heat mediated antigen retrieval step was performed prior to incubation with the antibody for 20 minutes. A biotinylated goat anti-rabbit antibody was used as the secondary.

    See Abreview

References for Anti-CD8 antibody (ab4055)

This product has been referenced in:
  • Chen S  et al. PD-L1 expression of the residual tumor serves as a prognostic marker in local advanced breast cancer after neoadjuvant chemotherapy. Int J Cancer 140:1384-1395 (2017). IHC-P ; Human . Read more (PubMed: 27925176) »
  • Tian W  et al. A prognostic risk model for patients with triple negative breast cancer based on stromal natural killer cells, tumor-associated macrophages and growth-arrest specific protein 6. Cancer Sci 107:882-9 (2016). IHC-P ; Human . Read more (PubMed: 27145494) »

See all 17 Publications for this product

Product Wall

I'm very sorry to hear you are having problems with ab833 and ab4055. I would like to suggest using triton (0.3%v/v) in the buffers used to dilute the primary and secondary antibodies and an overnight incubation. A 10min high temperature antigen unm...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Monkey Tissue sections (Spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Spleen
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted May 30 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Spleen
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted May 30 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
2.5% Normal Horse Serum - VectorLabs ImmPRESS™ Excel Amplified HRP Polymer Staining Kit (Anti-Mouse Ig) as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA pH 9.0 Bond Epitope Retrieval Solution 2 (ER2, Leica)
Sample
Rhesus monkey Tissue sections (Spleen)
Specification
Spleen
Permeabilization
No
Fixative
10% Neutral Buffered Formalin
Username

Abcam user community

Verified customer

Submitted Mar 26 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Diva Decloaker
Sample
Human Tissue sections (Intestine)
Specification
Intestine
Permeabilization
No
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 23 2014

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (4-20% gradient)
Sample
Dog Tissue lysate - whole (Spleen)
Specification
Spleen
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Sep 26 2014

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 23°C
Sample
Dog Tissue sections (Vastus Lateralis, Skeletal Muscle)
Specification
Vastus Lateralis, Skeletal Muscle
Permeabilization
Yes - 0.1% Triton in PBS
Fixative
OCT
Username

Abcam user community

Verified customer

Submitted Sep 08 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Sample
Human Tissue sections (Colon tumor)
Specification
Colon tumor
Permeabilization
Yes - PBS+0.3% Triton-X-100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Aug 13 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (lymph node)
Specification
lymph node
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Permeabilization
No
Blocking step
Dako blocking reagent as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Dr. R Berahovich

Verified customer

Submitted Mar 11 2013


For ab846, it is stated on the datasheet that antigen retrieval should be done at a high pH so pH9 would be suitable.

For ab4055 it is not specified what pH should be used so I would recommend heat mediated antigen retrieval either using...

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