• Product nameAnti-CD8 antibody [BL-Ts 8/2]
    See all CD8 primary antibodies
  • Description
    Mouse monoclonal [BL-Ts 8/2] to CD8
  • SpecificityThis antibody reacts with the alpha chain of CD8.
  • Tested applicationsSuitable for: IHC-Fr, Immunomicroscopy, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue/ cell preparation containing human CD8 protein.



Our Abpromise guarantee covers the use of ab34364 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notesFlow Cyt: Use at an assay dependent dilution.
    Note: ab34364 determination of CD4/CD8 ratio of T-lymphocytes, isolation of CD8+ T-cells by cellsorting or magnetic beads, and quantitative determination of CD8+ T-cells in blood.
    ICC/IF: Use at an assay dependent dilution.
    IHC-Fr: Use at an assay dependent dilution.
    Note: ab34364 can be used to identify CD8+ T-cells in tissue sections.
    IM: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionIdentifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
    • Involvement in diseaseDefects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
    • Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Post-translational
      All of the five most C-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
    • Cellular localizationSecreted and Cell membrane.
    • Information by UniProt
    • Database links
    • FormCD8 beta tissue specificity: Isoform 1, isoform 3, isoform 5, isoform 6, isoform 7 and isoform 8 are expressed in both thymus and peripheral CD8+ T-cells. Expression of isoform 1 is higher in thymus CD8+ T-cells than in peripheral CD8+ T-cells. Expression of isoform 6 is higher in peripheral CD8+ T-cells than in thymus CD8+ T-cells. CD8 beta PTM: Phosphorylated as a consequence of T-cell activation.
    • Alternative names
      • CD8 antibody
      • CD8 antigen, alpha polypeptide (p32) antibody
      • CD8a antibody
      • CD8a antigen antibody
      • CD8a molecule antibody
      • CD8A_HUMAN antibody
      • Leu2 antibody
      • Leu2 T lymphocyte antigen antibody
      • MAL antibody
      • OKT8 T cell antigen antibody
      • p32 antibody
      • T cell antigen Leu2 antibody
      • T cell co receptor antibody
      • T-cell surface glycoprotein CD8 alpha chain antibody
      • T-lymphocyte differentiation antigen T8/Leu-2 antibody
      • T8 T cell antigen antibody
      see all

    References for Anti-CD8 antibody [BL-Ts 8/2] (ab34364)

    This product has been referenced in:
    • Abdulamir AS  et al. Changing survival, memory cell compartment, and T-helper balance of lymphocytes between severe and mild asthma. BMC Immunol 9:73 (2008). ICC ; Human . Read more (PubMed: 19087256) »
    • Lührmann A  et al. Immunomodulating properties of the antibiotic novobiocin in human monocytes. Antimicrob Agents Chemother 42:1911-6 (1998). Read more (PubMed: 9687383) »

    See all 2 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Human Tissue sections (Brain)
    Specification Brain
    Fixative Acetone
    Permeabilization No
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 21°C

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    Submitted Jun 03 2011