Anti-CD8 antibody [MEM-31] (Allophycocyanin) (ab26004)

Overview

  • Product nameAnti-CD8 antibody [MEM-31] (Allophycocyanin)
    See all CD8 primary antibodies
  • Description
    Mouse monoclonal [MEM-31] to CD8 (Allophycocyanin)
  • ConjugationAllophycocyanin. Ex: 645nm, Em: 660nm
  • Tested applicationsSuitable for: Flow Cytmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue/ cell preparation: Crude thymus membrane fraction. (Human).

  • EpitopeThis antibody recognizes a conformationally dependent epitope of CD8 antigen.
  • General notes

    The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions.

Properties

Applications

Our Abpromise guarantee covers the use of ab26004 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. Use 5-20 µl per 100 µl of whole blood.



ab91364-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
  • Application notesIs unsuitable for WB.
  • Target

    • FunctionIdentifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
    • Involvement in diseaseDefects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
    • Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Post-translational
      modifications
      All of the five most C-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
    • Cellular localizationSecreted and Cell membrane.
    • Information by UniProt
    • Database links
    • FormCD8 beta tissue specificity: Isoform 1, isoform 3, isoform 5, isoform 6, isoform 7 and isoform 8 are expressed in both thymus and peripheral CD8+ T-cells. Expression of isoform 1 is higher in thymus CD8+ T-cells than in peripheral CD8+ T-cells. Expression of isoform 6 is higher in peripheral CD8+ T-cells than in thymus CD8+ T-cells. CD8 beta PTM: Phosphorylated as a consequence of T-cell activation.
    • Alternative names
      • CD8 antibody
      • CD8 antigen, alpha polypeptide (p32) antibody
      • CD8a antibody
      • CD8a antigen antibody
      • CD8a molecule antibody
      • CD8A_HUMAN antibody
      • Leu2 antibody
      • Leu2 T lymphocyte antigen antibody
      • MAL antibody
      • OKT8 T cell antigen antibody
      • p32 antibody
      • T cell antigen Leu2 antibody
      • T cell co receptor antibody
      • T-cell surface glycoprotein CD8 alpha chain antibody
      • T-lymphocyte differentiation antigen T8/Leu-2 antibody
      • T8 T cell antigen antibody
      see all

    References for Anti-CD8 antibody [MEM-31] (Allophycocyanin) (ab26004)

    This product has been referenced in:
    • Horejsí V  et al. Monoclonal antibodies against human leucocyte antigens. I. Antibodies against beta-2-microglobulin, immunoglobulin kappa light chains, HLA-DR-like antigens, T8 antigen, T1 antigen, a monocyte antigen, and a pan-leucocyte antigen. Folia Biol (Praha) 32:12-25 (1986). Read more (PubMed: 2422063) »

    See 1 Publication for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (Thymus)
    Specification Thymus
    Fixative Paraformaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer PH6.0
    Permeabilization No
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Username

    Dr. R. Teng

    Verified customer

    Submitted May 03 2012

    Thank you for submitting an Abreview of ab26004. As you probably have noticed, your review is going to bepublished very soon on our website.

    It was recently returned to you as we would like to know if the primary antibody was used diluted or ...

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    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (Thymus)
    Specification Thymus
    Fixative Acetone
    Permeabilization Yes - Triton X-100
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Username

    Dr. R. Teng

    Verified customer

    Submitted May 02 2012



    I am very pleased to hear you would like to accept our offer and testab18282, ab26004, and ab69764inIHC-P.You can just place the order for the 3 antibodies in the usual way you do it (through a purchaser, online, etc.). You do not need the co...

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    Thank you for your inquiry, which was passed along from my colleague.


    We do have the Multicolor Human T cell marker panel, but as you know it is only tested in and guaranteed for FC.


    http://www.abcam.com/Multicolor-Human...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"