SpecificityRecognizes the CD8 human T cytotoxic/suppressor lymphocyte cell surface glycoprotein. Approximately 90% of thymocytes are stained in suspension. In thymus sections, both cortical and medullary thymocytes are stained. A subset of NK cells express this antigen weakly.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesFlow Cyt: Use 5µl for 106 cells.
Monoclonal Anti-Human CD8 may be used for:
1. Enumeration of total T cytotoxic/suppressor lymphocytes in bone marrow, blood and other body fluids.
2. Identification and localization of T cytotoxic/suppressor lymphocytes in lymphoid and other tissues.
3. Analysis of cell mediated cytotoxicity.
4. Studies of immunoregulation in health and disease.
5. Investigation of NK cells.
6. Complement mediated cytolysis of CD8 expressing cells.
For fluorescence analysis of cells with Fc receptors, the use of isotype matched negative control is mandatory. In some systems it may be necessary to incubate the cells in 10-20% normal serum from the second antibody host species (at step 2 before adding monoclonal antibody) in order to decrease non-specific staining with
the conjugated second antibody.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionIdentifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
Involvement in diseaseDefects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
FormCD8 beta tissue specificity: Isoform 1, isoform 3, isoform 5, isoform 6, isoform 7 and isoform 8 are expressed in both thymus and peripheral CD8+ T-cells. Expression of isoform 1 is higher in thymus CD8+ T-cells than in peripheral CD8+ T-cells. Expression of isoform 6 is higher in peripheral CD8+ T-cells than in thymus CD8+ T-cells.
CD8 beta PTM: Phosphorylated as a consequence of T-cell activation.