Anti-CD8 antibody [YTC182.20] (ab60076)

Overview

  • Product nameAnti-CD8 antibody [YTC182.20]
    See all CD8 primary antibodies
  • Description
    Rat monoclonal [YTC182.20] to CD8
  • SpecificityRecognizes the human CD8 cell surface antigen
  • Tested applicationsSuitable for: IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Human, Monkey, Cynomolgus Monkey, Rhesus monkey
  • Immunogen

    Mouse L cells transfected with human CD8 gene.

  • Positive control
    • Human peripheral blood lymphocytes.

Properties

Applications

Our Abpromise guarantee covers the use of ab60076 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
Flow Cyt 1/100 - 1/200.

Use 10µl of the suggested working dilution to label 106 cells in 100µl.
ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Target

  • FunctionIdentifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
  • Involvement in diseaseDefects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
  • Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    All of the five most C-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • FormCD8 beta tissue specificity: Isoform 1, isoform 3, isoform 5, isoform 6, isoform 7 and isoform 8 are expressed in both thymus and peripheral CD8+ T-cells. Expression of isoform 1 is higher in thymus CD8+ T-cells than in peripheral CD8+ T-cells. Expression of isoform 6 is higher in peripheral CD8+ T-cells than in thymus CD8+ T-cells. CD8 beta PTM: Phosphorylated as a consequence of T-cell activation.
  • Alternative names
    • CD8 antibody
    • CD8 antigen, alpha polypeptide (p32) antibody
    • CD8a antibody
    • CD8a antigen antibody
    • CD8a molecule antibody
    • CD8A_HUMAN antibody
    • Leu2 antibody
    • Leu2 T lymphocyte antigen antibody
    • MAL antibody
    • OKT8 T cell antigen antibody
    • p32 antibody
    • T cell antigen Leu2 antibody
    • T cell co receptor antibody
    • T-cell surface glycoprotein CD8 alpha chain antibody
    • T-lymphocyte differentiation antigen T8/Leu-2 antibody
    • T8 T cell antigen antibody
    see all

Anti-CD8 antibody [YTC182.20] images

  • ab60076 at 1/100 dilution staining CD8 in human peripheral blood lymphocytes by Flow Cytometry.

References for Anti-CD8 antibody [YTC182.20] (ab60076)

ab60076 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Sample Cynomolgus Monkey Tissue sections (Lung)
Specification Lung
Permeabilization No
Fixative Formaldehyde
Username

Dr. Francesca Polverino

Verified customer

Submitted Oct 15 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"