The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use 0.5µg for 106 cells.
IHC-P: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 24 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing.
Isoform 1, isoform 3, isoform 5, isoform 6, isoform 7 and isoform 8 are expressed in both thymus and peripheral CD8+ T-cells. Expression of isoform 1 is higher in thymus CD8+ T-cells than in peripheral CD8+ T-cells. Expression of isoform 6 is higher in peripheral CD8+ T-cells than in thymus CD8+ T-cells.
Staining of Lou rat splenocytes with 0.25 µg of Purified Mouse IgG1, K Isotype Control (open histogram) or 0.25 µg of ab90965 (colored histogram) followed by Biotin Anti-Mouse IgG. Cells in the lymphocyte gate were used for analysis.
Hirji N et al. Mechanisms of macrophage stimulation through CD8: macrophage CD8alpha and CD8beta induce nitric oxide production and associated killing of the parasite Leishmania major. J Immunol160:6004-11 (1998).
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