Overview

  • Product nameAnti-CD8 beta antibody [341]
    See all CD8 beta primary antibodies
  • Description
    Mouse monoclonal [341] to CD8 beta
  • Tested applicationsSuitable for: IHC-P, Flow Cyt, IP, WBmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Rat/mouse T cell hybrids expressing CD8.

  • Positive control
    • Lou rat splenocytes and thymocytes.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone number341
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab90965 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Flow Cyt
IP
WB
  • Application notesFlow Cyt: Use 0.5µg for 106 cells.
    IHC-P: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 24 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionIdentifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing.
    • Tissue specificityIsoform 1, isoform 3, isoform 5, isoform 6, isoform 7 and isoform 8 are expressed in both thymus and peripheral CD8+ T-cells. Expression of isoform 1 is higher in thymus CD8+ T-cells than in peripheral CD8+ T-cells. Expression of isoform 6 is higher in peripheral CD8+ T-cells than in thymus CD8+ T-cells.
    • Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Post-translational
      modifications
      Phosphorylated as a consequence of T-cell activation.
    • Cellular localizationSecreted and Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD8b antigen antibody
      • CD8 antigen beta polypeptide 1 (p37) antibody
      • CD8 antigen beta polypeptide 1 antibody
      • CD8 beta antibody
      • CD8b antibody
      • CD8b molecule antibody
      • CD8B_HUMAN antibody
      • CD8B1 antibody
      • Leu2 antibody
      • Ly3 antibody
      • LYT3 antibody
      • MGC119115 antibody
      • P37 antibody
      • T cell surface glycoprotein CD8 beta chain antibody
      • T lymphocyte surface glycoprotein beta chain antibody
      • T-cell surface glycoprotein CD8 beta chain antibody
      see all

    Anti-CD8 beta antibody [341] images

    • Staining of Lou rat splenocytes with 0.25 µg of Purified Mouse IgG1, K Isotype Control (open histogram) or 0.25 µg of ab90965 (colored histogram) followed by Biotin Anti-Mouse IgG. Cells in the lymphocyte gate were used for analysis.

    References for Anti-CD8 beta antibody [341] (ab90965)

    This product has been referenced in:
    • Hirji N  et al. Mechanisms of macrophage stimulation through CD8: macrophage CD8alpha and CD8beta induce nitric oxide production and associated killing of the parasite Leishmania major. J Immunol 160:6004-11 (1998). Read more (PubMed: 9637515) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab90965.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"