Overview

  • Product name
    Anti-CD8 beta antibody [341]
    See all CD8 beta primary antibodies
  • Description
    Mouse monoclonal [341] to CD8 beta
  • Tested applications
    Suitable for: IHC-P, Flow Cyt, IP, WBmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Rat/mouse T cell hybrids expressing CD8.

  • Positive control
    • Lou rat splenocytes and thymocytes.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    341
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab90965 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Flow Cyt
IP
WB
  • Application notes
    Flow Cyt: Use 0.5µg for 106 cells.
    IHC-P: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 24 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing.
    • Tissue specificity
      Isoform 1, isoform 3, isoform 5, isoform 6, isoform 7 and isoform 8 are expressed in both thymus and peripheral CD8+ T-cells. Expression of isoform 1 is higher in thymus CD8+ T-cells than in peripheral CD8+ T-cells. Expression of isoform 6 is higher in peripheral CD8+ T-cells than in thymus CD8+ T-cells.
    • Sequence similarities
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Post-translational
      modifications
      Phosphorylated as a consequence of T-cell activation.
    • Cellular localization
      Secreted and Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD8b antigen antibody
      • CD8 antigen beta polypeptide 1 (p37) antibody
      • CD8 antigen beta polypeptide 1 antibody
      • CD8 beta antibody
      • CD8b antibody
      • CD8b molecule antibody
      • CD8B_HUMAN antibody
      • CD8B1 antibody
      • Leu2 antibody
      • Ly3 antibody
      • LYT3 antibody
      • MGC119115 antibody
      • P37 antibody
      • T cell surface glycoprotein CD8 beta chain antibody
      • T lymphocyte surface glycoprotein beta chain antibody
      • T-cell surface glycoprotein CD8 beta chain antibody
      see all

    Images

    • Staining of Lou rat splenocytes with 0.25 µg of Purified Mouse IgG1, K Isotype Control (open histogram) or 0.25 µg of ab90965 (colored histogram) followed by Biotin Anti-Mouse IgG. Cells in the lymphocyte gate were used for analysis.

    References

    This product has been referenced in:
    • Hirji N  et al. Mechanisms of macrophage stimulation through CD8: macrophage CD8alpha and CD8beta induce nitric oxide production and associated killing of the parasite Leishmania major. J Immunol 160:6004-11 (1998). Read more (PubMed: 9637515) »

    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab90965.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up