Overview

  • Product name
    Anti-CD8 beta antibody [H35-17.2]
    See all CD8 beta primary antibodies
  • Description
    Rat monoclonal [H35-17.2] to CD8 beta
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    5-day mixed leukocyte culture population, C57BL/6 anti-BALB/c

  • Positive control
    • BALB/c splenocytes; Mouse splenocytes and thymocytes

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: Less than or equal to 0.09% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    H35-17.2
  • Isotype
    IgG2b
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab95768 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.25-0.5µg for 105-8 cells. Staining the cell sample in a final volume of 100 µl is recommended.



ab18536-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing.
  • Tissue specificity
    Isoform 1, isoform 3, isoform 5, isoform 6, isoform 7 and isoform 8 are expressed in both thymus and peripheral CD8+ T-cells. Expression of isoform 1 is higher in thymus CD8+ T-cells than in peripheral CD8+ T-cells. Expression of isoform 6 is higher in peripheral CD8+ T-cells than in thymus CD8+ T-cells.
  • Sequence similarities
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    Phosphorylated as a consequence of T-cell activation.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD8b antigen antibody
    • CD8 antigen beta polypeptide 1 (p37) antibody
    • CD8 antigen beta polypeptide 1 antibody
    • CD8 beta antibody
    • CD8b antibody
    • CD8b molecule antibody
    • CD8B_HUMAN antibody
    • CD8B1 antibody
    • Leu2 antibody
    • Ly3 antibody
    • LYT3 antibody
    • MGC119115 antibody
    • P37 antibody
    • T cell surface glycoprotein CD8 beta chain antibody
    • T lymphocyte surface glycoprotein beta chain antibody
    • T-cell surface glycoprotein CD8 beta chain antibody
    see all

Anti-CD8 beta antibody [H35-17.2] images

  • Flow cytometry staining of BALB/c splenocytes with 0.25 µg of Rat IgG2b Isotype Control (open histogram) or 0.25 µg of ab95768 (filled histogram) followed by Anti-Rat IgG FITC. Cells in the lymphocyte gate were used for analysis.

References for Anti-CD8 beta antibody [H35-17.2] (ab95768)

This product has been referenced in:
  • Tarleton RL  et al. Depletion of T-cell subpopulations results in exacerbation of myocarditis and parasitism in experimental Chagas' disease. Infect Immun 62:1820-9 (1994). Read more (PubMed: 8168945) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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