Overview

  • Product nameAnti-CD8 beta antibody [H35-17.2] (PE/Cy5®)
    See all CD8 beta primary antibodies
  • Description
    Rat monoclonal [H35-17.2] to CD8 beta (PE/Cy5®)
  • ConjugationPE/Cy5®. Ex: 496nm, Em: 670nm
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    5-day mixed leukocyte culture population, C57BL/6 anti-BALB/c

  • Positive control
    • C57Bl/6 splenocytes

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone numberH35-17.2
  • IsotypeIgG2b
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab95769 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.125µg for 105-8 cells.
Recommended final staining volume is 100µl.

Target

  • FunctionIdentifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing.
  • Tissue specificityIsoform 1, isoform 3, isoform 5, isoform 6, isoform 7 and isoform 8 are expressed in both thymus and peripheral CD8+ T-cells. Expression of isoform 1 is higher in thymus CD8+ T-cells than in peripheral CD8+ T-cells. Expression of isoform 6 is higher in peripheral CD8+ T-cells than in thymus CD8+ T-cells.
  • Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    Phosphorylated as a consequence of T-cell activation.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD8b antigen antibody
    • CD8 antigen beta polypeptide 1 (p37) antibody
    • CD8 antigen beta polypeptide 1 antibody
    • CD8 beta antibody
    • CD8b antibody
    • CD8b molecule antibody
    • CD8B_HUMAN antibody
    • CD8B1 antibody
    • Leu2 antibody
    • Ly3 antibody
    • LYT3 antibody
    • MGC119115 antibody
    • P37 antibody
    • T cell surface glycoprotein CD8 beta chain antibody
    • T lymphocyte surface glycoprotein beta chain antibody
    • T-cell surface glycoprotein CD8 beta chain antibody
    see all

Anti-CD8 beta antibody [H35-17.2] (PE/Cy5®) images

  • Staining of C57Bl/6 splenocytes with Anti-Mouse CD8a FITC and ab95769 at 0.06 µg. Cells in the lymphocyte gate were used for analysis.

References for Anti-CD8 beta antibody [H35-17.2] (PE/Cy5®) (ab95769)

This product has been referenced in:
  • Thoma-Uszynski S  et al. CD8alphaalpha T cells in lesions of Listeria monocytogenes-infected beta2m-deficient mice. Microb Pathog 23:101-6 (1997). IHC-Fr . Read more (PubMed: 9245621) »
  • Tarleton RL  et al. Depletion of T-cell subpopulations results in exacerbation of myocarditis and parasitism in experimental Chagas' disease. Infect Immun 62:1820-9 (1994). IHC-Fr . Read more (PubMed: 8168945) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"