Anti-CD8 + HLA Class II antibody [733 + LN3] (Dual color: FITC + PE) (ab1236)

Overview

  • Product nameAnti-CD8 + HLA Class II antibody [733 + LN3] (Dual color: FITC + PE)
  • Description
    Mouse monoclonal [733 + LN3] to CD8 + HLA Class II (Dual color: FITC + PE)
  • ConjugationDual color: FITC + PE
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • General notesThis product is a mixture of two clones: CD8 = Clone:733, Isotype:IgG1, Label:FITC HLA-DR = Clone:LN3, Isotype:IgG2b, Label:PE This product contains sodium azide, which under acid conditions yields hydrazoic acid, a toxic compound. Azide compounds should be diluted with running water before being discarded to avoid deposits in lead or copper plumbing where explosive conditions may develop.

Properties

Applications

Our Abpromise guarantee covers the use of ab1236 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notesCounting and identification of CD8+ T lymphocytes in human lysed whole peripheral blood or mononuclear cells separated by density gradient.
    Percentage count of CD8 activated lymphocytes.
    Studies of HIV (Human Immunodeficiency Virus) infection.

    CD8(FITC) / HLA-DR (PE) immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers.

    10µl ofCD8(FITC) / HLA-DR (PE) is sufficient for labelling 1x106 cells.
  • Target

    • RelevanceCD8: This antigen is present on human T suppressor/cytotoxic cell subtypes, that represents 20-36% of normal peripheral blood lymphocytes, and on NK cells subset (CD3-CD8+) and thymocytes. Patients with AIDS exhibit T cells lymphopenia, a loss of CD4+ lymphocytes and a relative increase in the CD8+ subtype and in the CD3+CD4-CD8- subtype. HLA-DR (human Class II histocompatibility antigen): This antigen is present on antigen presenting cells (APC), B cells, monocytes, thymic epithelial cells and macrophages. It is also expressed on activated T cells, but not on granulocytes, platelets or red blood cells. Resting T cells or endothelial cells can be induced to express HLA-DR antigens.
    • Cellular localizationMembrane; singlepass type I membrane protein.
    • Database links

    References for Anti-CD8 + HLA Class II antibody [733 + LN3] (Dual color: FITC + PE) (ab1236)

    ab1236 has not yet been referenced specifically in any publications.

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