Anti-CD80 antibody [16-10A1] (FITC) (ab95550)

Overview

  • Product nameAnti-CD80 antibody [16-10A1] (FITC)
    See all CD80 primary antibodies
  • Description
    Armenian Hamster monoclonal [16-10A1] to CD80 (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • SpecificityReacts with mouse CD80, a 55 kDa member of the Ig superfamily.
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    CHO cell line stably transfected with mouse B7 (Cd80) cDNA

  • Positive control
    • C57BL/6 splenocytes

Properties

Applications

Our Abpromise guarantee covers the use of ab95550 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.25µg for 105 cells. Cell number should be determined empirically but can range from 105 to 108 cells/test (based on 100 µl test volume).



ab18473-Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • FunctionInvolved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.
  • Tissue specificityExpressed on activated B-cells, macrophages and dendritic cells.
  • Sequence similaritiesContains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activation B7-1 antigen antibody
    • B lymphocyte activation antigen B7 antibody
    • B7 antibody
    • B7-1 antibody
    • B7-1 antigen antibody
    • B7.1 antibody
    • BB1 antibody
    • CD28 antigen ligand 1 antibody
    • CD28LG antibody
    • CD28LG1 antibody
    • CD80 antibody
    • CD80 antigen (CD28 antigen ligand 1, B7-1 antigen) antibody
    • CD80 antigen antibody
    • CD80 molecule antibody
    • CD80_HUMAN antibody
    • Costimulatory factor CD80 antibody
    • costimulatory molecule variant IgV-CD80 antibody
    • CTLA-4 counter-receptor B7.1 antibody
    • LAB7 antibody
    • T-lymphocyte activation antigen CD80 antibody
    see all

Anti-CD80 antibody [16-10A1] (FITC) images

  • Staining of: (1) unstimulated C57BL/6 splenocytes and (2) 3 day LPS stimulated C57BL/6 splenocytes with ab95550 at 0.125 µg. Total viable cells were used for analysis. Markers were set based on the autofluorescence sample.

References for Anti-CD80 antibody [16-10A1] (FITC) (ab95550)

ab95550 has not yet been referenced specifically in any publications.

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