Anti-CD80 antibody [1G10] (Allophycocyanin) (ab25555)

Overview

  • Product nameAnti-CD80 antibody [1G10] (Allophycocyanin)
    See all CD80 primary antibodies
  • Description
    Rat monoclonal [1G10] to CD80 (Allophycocyanin)
  • ConjugationAllophycocyanin. Ex: 645nm, Em: 660nm
  • Tested applicationsSuitable for: Flow Cyt, IP, Blockingmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    The details of the immunogen for this antibody are not available.

Properties

Applications

Our Abpromise guarantee covers the use of ab25555 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.2µg for 106 cells.
IP Use at an assay dependent dilution.
Blocking Use at an assay dependent dilution. Can be used for in vivo and in vitro blocking of ligand binding. The antibody does not block stimulation of T cells by natural antigen presenting cells.

Target

  • FunctionInvolved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.
  • Tissue specificityExpressed on activated B-cells, macrophages and dendritic cells.
  • Sequence similaritiesContains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activation B7-1 antigen antibody
    • B lymphocyte activation antigen B7 antibody
    • B7 antibody
    • B7-1 antibody
    • B7-1 antigen antibody
    • B7.1 antibody
    • BB1 antibody
    • CD28 antigen ligand 1 antibody
    • CD28LG antibody
    • CD28LG1 antibody
    • CD80 antibody
    • CD80 antigen (CD28 antigen ligand 1, B7-1 antigen) antibody
    • CD80 antigen antibody
    • CD80 molecule antibody
    • CD80_HUMAN antibody
    • Costimulatory factor CD80 antibody
    • costimulatory molecule variant IgV-CD80 antibody
    • CTLA-4 counter-receptor B7.1 antibody
    • LAB7 antibody
    • T-lymphocyte activation antigen CD80 antibody
    see all

References for Anti-CD80 antibody [1G10] (Allophycocyanin) (ab25555)

ab25555 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (Spleen cells)
Permeabilization No
Gating Strategy CD45+ CD11c+ CD80+
Specification Spleen cells
Preparation Cell harvesting/tissue preparation method: Spleens were smushed through a 70um filter, then RBCs were lysed
Sample buffer: PBS + 2% FBS
Fixation Formaldehyde
Username

Ms. Cheryl Sandoval

Verified customer

Submitted Jun 28 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"