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The details of the immunogen for this antibody are not available.
Our Abpromise guarantee covers the use of ab24860 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.
ab18446 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|Functional Studies||Use at an assay dependent concentration.
Also suitable for in vivo and in vitro blocking of ligand binding.
|IP||Use at an assay dependent concentration.|
P815 cells transfected with CD80 or non-transfected P815 control cells were stained with ab24860 (3 µg/106 cells). Large cells were then gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA).
ab24860 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"