Anti-CD80 antibody [2A2] (ab86473)

Overview

  • Product nameAnti-CD80 antibody [2A2]
    See all CD80 primary antibodies
  • Description
    Mouse monoclonal [2A2] to CD80
  • Tested applicationsSuitable for: IHC-P, WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified recombinant fragment of Human CD80 expressed in E. coli spanning amino acids 35-141.

  • Positive control
    • BCBL-1 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab86473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/1000.
WB 1/500 - 1/2000. Predicted molecular weight: 33 kDa.
ELISA 1/10000.
ICC/IF 1/200 - 1/1000.

Target

  • FunctionInvolved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.
  • Tissue specificityExpressed on activated B-cells, macrophages and dendritic cells.
  • Sequence similaritiesContains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activation B7-1 antigen antibody
    • B lymphocyte activation antigen B7 antibody
    • B7 antibody
    • B7-1 antibody
    • B7-1 antigen antibody
    • B7.1 antibody
    • BB1 antibody
    • CD28 antigen ligand 1 antibody
    • CD28LG antibody
    • CD28LG1 antibody
    • CD80 antibody
    • CD80 antigen (CD28 antigen ligand 1, B7-1 antigen) antibody
    • CD80 antigen antibody
    • CD80 molecule antibody
    • CD80_HUMAN antibody
    • Costimulatory factor CD80 antibody
    • costimulatory molecule variant IgV-CD80 antibody
    • CTLA-4 counter-receptor B7.1 antibody
    • LAB7 antibody
    • T-lymphocyte activation antigen CD80 antibody
    see all

Anti-CD80 antibody [2A2] images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD80 with ab86473.

  • Confocal immunofluorescence analysis of BCBL-1 cells using ab86473 at 1/200 dilution (green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.

References for Anti-CD80 antibody [2A2] (ab86473)

ab86473 has not yet been referenced specifically in any publications.

Product Wall



ab86473: endotoxin level was not measured


ab130164: endotoxin level of the antibody solution was not determined, but the purification process was performed in a clean environment, thus we assume the endotoxin level is quite low....

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