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Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse CD80.
(Peptide available as ab66476.)
Our Abpromise guarantee covers the use of ab64116 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 33, 65 kDa (predicted molecular weight: 33 kDa).|
|IHC-P||1/40. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
ab64116 (1:40) staining CD80 in paraffin-embedded mouse lymph node tissue (left panel) using an automated system (Ventana Discovery). Right-hand panel shows negative control (no primary antibody).
Using this protocol there is strong membrane staining, of activated B cells in the germinal centres. What appear to be scattered activated B cells or possibly plasma cells in the interfollicular areas of the lymph node and blood vessels.
Sections were rehydrated and antigen retrieved in CC1 Cell Conditioning Buffer using Ventana Extended Retrieval programme. Slides were blocked in 3% H2O2 / 4 min / 37°C and incubated with ab64116 (1:40 dilution / 1 hour / 37°C). Sections then blocked (4mins / 37°C) and incubated with Dako swine anti-rabbit antibody (1:50, 28 min / 37°C). Staining was amplified and detected by incubation with Ventana Streptavidin ABC system (16 min / 37°C) and Ventana DAB map reagent (8
CD80 has a predicted molecular weight of 33 kDa, however it is predicted to be extensively glycosylated (SwissProt data). The band seen at 65 kDa is consistent with the banding pattern observed for other commercially available antibodies to CD80. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"