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Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse CD80.
(Peptide available as ab66476.)
Our Abpromise guarantee covers the use of ab64116 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/40. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
ab64116 (1:40) staining CD80 in paraffin-embedded mouse lymph node tissue (left panel) using an automated system (Ventana Discovery). Right-hand panel shows negative control (no primary antibody).
Using this protocol there is strong membrane staining, of activated B cells in the germinal centres. What appear to be scattered activated B cells or possibly plasma cells in the interfollicular areas of the lymph node and blood vessels.
Sections were rehydrated and antigen retrieved in CC1 Cell Conditioning Buffer using Ventana Extended Retrieval programme. Slides were blocked in 3% H2O2 / 4 min / 37°C and incubated with ab64116 (1:40 dilution / 1 hour / 37°C). Sections then blocked (4mins / 37°C) and incubated with Dako swine anti-rabbit antibody (1:50, 28 min / 37°C). Staining was amplified and detected by incubation with Ventana Streptavidin ABC system (16 min / 37°C) and Ventana DAB map reagent (8
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