Overview

  • Product nameAnti-CD82 antibody [B-L2]
    See all CD82 primary antibodies
  • Description
    Mouse monoclonal [B-L2] to CD82
  • Tested applicationsSuitable for: IHC-P, IHC-Fr, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    CD82 transfected P815 cell line

Properties

Applications

Our Abpromise guarantee covers the use of ab47153 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionAssociates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway.
  • Tissue specificityLymphoid specific.
  • Sequence similaritiesBelongs to the tetraspanin (TM4SF) family.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4F9 antibody
    • Antigen detected by monoclonal and antibody IA4 antibody
    • C33 antibody
    • C33 antigen antibody
    • CD 82 antibody
    • CD82 antibody
    • CD82 antigen antibody
    • CD82 molecule antibody
    • CD82_HUMAN antibody
    • GR 15 antibody
    • GR15 antibody
    • IA 4 antibody
    • IA4 antibody
    • Inducible membrane protein antibody
    • Inducible membrane protein R2 antibody
    • KAI 1 antibody
    • KAI1 antibody
    • Kangai 1 (suppression of tumorigenicity 6, prostate; CD82 antigen (R2 leukocyte antigen, antigen detected by monoclonal and antibody IA4)) antibody
    • Kangai 1 antibody
    • Kangai1 antibody
    • Leukocyte surface antigen R2 antibody
    • Metastasis suppressor Kangai 1 antibody
    • Metastasis suppressor Kangai-1 antibody
    • Metastasis suppressor Kangai1 antibody
    • Prostate cancer antimetastasis gene KAI1 antibody
    • R2 antibody
    • R2 leukocyte antigen antibody
    • SAR 2 antibody
    • SAR2 antibody
    • ST 6 antibody
    • ST6 antibody
    • Suppression of tumorigenicity 6 antibody
    • Suppression of tumorigenicity 6 prostate antibody
    • Suppressor of tumorigenicity 6 antibody
    • Suppressor of tumorigenicity 6 protein antibody
    • Tetraspanin 27 antibody
    • Tetraspanin-27 antibody
    • Tetraspanin27 antibody
    • Tspan 27 antibody
    • Tspan-27 antibody
    • Tspan27 antibody
    see all

Anti-CD82 antibody [B-L2] images

  • A typical staining pattern with the B-L2 monoclonal antibody of lymphocytes
  • ab47153 staining HeLa cells.

    ICC/IF was performed with a standard paraformaldehyde technique (non permeabilized, blocked with 0.2% fish gelatin for 30 min at 25°C). Primary antibody used at 1/200 in 0.12% PBS / fish gelatin for one hour.  The secondary antibody was a Cy3-conjugated Goat anti-Mouse. 

    See Abreview

  • IHC image of ab47153 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab47153, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-CD82 antibody [B-L2] (ab47153)

This product has been referenced in:
  • Artavanis-Tsakonas K  et al. The Tetraspanin CD82 is Specifically Recruited to Fungal and Bacterial Phagosomes Prior to Acidification. Infect Immun : (2010). ICC/IF ; Human . Read more (PubMed: 21149584) »

See 1 Publication for this product

Product Wall


I am sorry to confirm that the epitope for these antibodies have not been mapped. This is not often done for research grade antibodies.

I can confirm that the immunisation was done with transfected cells. So the immunogen was the full seq...

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Thank you for your reply.


We currently only have GR45737-2 and GR45737-3 in stock. We have sold out of GR45737-1. However, these are all the same antibody and are all from the same parent lot, just aliquoted at different times. If you w...

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Thank you for your inquiry.


Unfortunately, we have not tested, nor do we guarantee, this antibody to work in blocking protein-protein interactions. If we had, we would list that on the online datasheet as "blocking," "functional studies...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa cells)
Specification HeLa cells
Fixative Paraformaldehyde
Permeabilization No
Blocking step fish gelatin as blocking agent for 30 minute(s) · Concentration: 0.2% · Temperature: 25°C
Username

Dr. Lydia Danglot

Verified customer

Submitted Nov 13 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"