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Our Abpromise guarantee covers the use of ab47153 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IHC-Fr||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
ab47153 staining HeLa cells.
ICC/IF was performed with a standard paraformaldehyde technique (non permeabilized, blocked with 0.2% fish gelatin for 30 min at 25°C). Primary antibody used at 1/200 in 0.12% PBS / fish gelatin for one hour. The secondary antibody was a Cy3-conjugated Goat anti-Mouse.