Overview

  • Product nameAnti-CD82 antibody [EPR4112]
    See all CD82 primary antibodies
  • Description
    Rabbit monoclonal [EPR4112] to CD82
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC or IP
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to a region within the C-terminal end of the intracellular domain of Human CD82.

  • Positive control
    • U87 MG, Jurkat, Raji and C6 cell lysates; Human tonsil tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab109529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 30 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notesIs unsuitable for ICC or IP.
  • Target

    • FunctionAssociates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway.
    • Tissue specificityLymphoid specific.
    • Sequence similaritiesBelongs to the tetraspanin (TM4SF) family.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 4F9 antibody
      • Antigen detected by monoclonal and antibody IA4 antibody
      • C33 antibody
      • C33 antigen antibody
      • CD 82 antibody
      • CD82 antibody
      • CD82 antigen antibody
      • CD82 molecule antibody
      • CD82_HUMAN antibody
      • GR 15 antibody
      • GR15 antibody
      • IA 4 antibody
      • IA4 antibody
      • Inducible membrane protein antibody
      • Inducible membrane protein R2 antibody
      • KAI 1 antibody
      • KAI1 antibody
      • Kangai 1 (suppression of tumorigenicity 6, prostate; CD82 antigen (R2 leukocyte antigen, antigen detected by monoclonal and antibody IA4)) antibody
      • Kangai 1 antibody
      • Kangai1 antibody
      • Leukocyte surface antigen R2 antibody
      • Metastasis suppressor Kangai 1 antibody
      • Metastasis suppressor Kangai-1 antibody
      • Metastasis suppressor Kangai1 antibody
      • Prostate cancer antimetastasis gene KAI1 antibody
      • R2 antibody
      • R2 leukocyte antigen antibody
      • SAR 2 antibody
      • SAR2 antibody
      • ST 6 antibody
      • ST6 antibody
      • Suppression of tumorigenicity 6 antibody
      • Suppression of tumorigenicity 6 prostate antibody
      • Suppressor of tumorigenicity 6 antibody
      • Suppressor of tumorigenicity 6 protein antibody
      • Tetraspanin 27 antibody
      • Tetraspanin-27 antibody
      • Tetraspanin27 antibody
      • Tspan 27 antibody
      • Tspan-27 antibody
      • Tspan27 antibody
      see all

    Anti-CD82 antibody [EPR4112] images

    • All lanes : Anti-CD82 antibody [EPR4112] (ab109529) at 1/1000 dilution

      Lane 1 : U87 MG cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : Raji cell lysate
      Lane 4 : C6 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 30 kDa
    • ab109529, at 1/250 dilution, staining CD82, in paraffin embedded Human tonsil tissue by Immunohistochemistry.

    References for Anti-CD82 antibody [EPR4112] (ab109529)

    This product has been referenced in:
    • Ding C  et al. Long non-coding RNA HOTAIR promotes cell migration and invasion via down-regulation of RNA binding motif protein 38 in hepatocellular carcinoma cells. Int J Mol Sci 15:4060-76 (2014). WB ; Human . Read more (PubMed: 24663081) »

    See 1 Publication for this product

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