Overview

  • Product nameAnti-CD82 antibody [TS82b]
    See all CD82 primary antibodies
  • Description
    Mouse monoclonal [TS82b] to CD82
  • Tested applicationsIHC-P, WB, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Jurkat and HEL cell lines.

  • Positive control
    • HEL cells

Properties

Applications

Our Abpromise guarantee covers the use of ab59509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Use under non reducing condition. Predicted molecular weight: 37 kDa.
IP Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells.

Target

  • FunctionAssociates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway.
  • Tissue specificityLymphoid specific.
  • Sequence similaritiesBelongs to the tetraspanin (TM4SF) family.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4F9 antibody
    • Antigen detected by monoclonal and antibody IA4 antibody
    • C33 antibody
    • C33 antigen antibody
    • CD 82 antibody
    • CD82 antibody
    • Cd82 antibody
    • CD82 antigen antibody
    • CD82 molecule antibody
    • CD82_HUMAN antibody
    • GR 15 antibody
    • GR15 antibody
    • IA 4 antibody
    • IA4 antibody
    • Inducible membrane protein antibody
    • Inducible membrane protein R2 antibody
    • KAI 1 antibody
    • KAI1 antibody
    • Kangai 1 (suppression of tumorigenicity 6, prostate; CD82 antigen (R2 leukocyte antigen, antigen detected by monoclonal and antibody IA4)) antibody
    • Kangai 1 antibody
    • Kangai1 antibody
    • Leukocyte surface antigen R2 antibody
    • Metastasis suppressor Kangai 1 antibody
    • Metastasis suppressor Kangai-1 antibody
    • Metastasis suppressor Kangai1 antibody
    • Prostate cancer antimetastasis gene KAI1 antibody
    • R2 antibody
    • R2 leukocyte antigen antibody
    • SAR 2 antibody
    • SAR2 antibody
    • ST 6 antibody
    • ST6 antibody
    • Suppression of tumorigenicity 6 antibody
    • Suppression of tumorigenicity 6 prostate antibody
    • Suppressor of tumorigenicity 6 antibody
    • Suppressor of tumorigenicity 6 protein antibody
    • Tetraspanin 27 antibody
    • Tetraspanin-27 antibody
    • Tetraspanin27 antibody
    • Tspan 27 antibody
    • Tspan-27 antibody
    • Tspan27 antibody
    see all

Anti-CD82 antibody [TS82b] images

  • ab59509 staining CD82 in Rat placenta tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilsed with 0.3% Triton X-100 and blocked with 3% serum for 60 minutes at 23°C; antigen retrieval was by heat mediation in 10mM citrate buffer pH6.0. Samples were incubated with primary antibody (1/200 in blocking buffer) for 12 hours at 4°C. A Biotin-conjugated Goat anti-mouse IgG polyclonal (1/100) was used as the secondary antibody.

    See Abreview

  • ab59509 staining CD82 (left) in Human peripheral blood neutrophils by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 1% BSA for 30 minutes at 22°C. Samples were incubated with primary antibody (1/100 in PBS + 0.1% Triton-X 100 + 1% BSA) for 12 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody. Middle - LFA 1a, Right - merge.

    See Abreview



  • Predicted band size : 37 kDa
    1.3X105 HEL cells were lysed by the following lysis buffer: 10mM Tris, pH 7.4, 150mM NaCl, 1mM CaCl2, 1mM MgCl2, 0.02% NaN3, 1mM PMSF, 0.5 lg/ml leupeptin, 1 lg/ml pepstatin A, and 10 KIU/ml aprotinin containing 1% detergent (Brij 97 or Triton). The lysate was separated by SDS/PAGE (5–15% gel) under non-reducing conditions and transferred to a PVDF membrane. ab59509 incubation time was 1h. 1,3 105 HEL cells were lysed (lysis buffer containing 1%Brij97 or triton) Migration SDS/page 5-15% gel under non-reducing conditions.
  • Overlay histogram showing HeLa cells stained with ab59509 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59509, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  • Overlay histogram showing HeLa cells stained with ab59509 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59509, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  • Overlay histogram showing HeLa cells stained with ab59509 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59509, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG, H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

References for Anti-CD82 antibody [TS82b] (ab59509)

This product has been referenced in:
  • Spring FA  et al. Tetraspanins CD81 and CD82 facilitate a4ß1-mediated adhesion of human erythroblasts to vascular cell adhesion molecule-1. PLoS One 8:e62654 (2013). IP ; Human . Read more (PubMed: 23704882) »
  • Saleh SM  et al. Identification of the tetraspanin CD82 as a new barrier to xenotransplantation. J Immunol 191:2796-805 (2013). Read more (PubMed: 23872050) »

See all 3 Publications for this product

Product Wall

Application Flow Cytometry
Fixation Paraformaldehyde
Permeabilization Yes - eBioscience Permeabilization Buffer
Sample Human Cell (Patient Dermal Fibroblasts)
Specification Patient Dermal Fibroblasts
Gating Strategy Isotype negative control (white)
Preparation Cell harvesting/tissue preparation method: Typsin-EDTA Cell Dissociation
Sample buffer: PBS and 10% FBS
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Submitted Feb 05 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer (10 mM citrate sodium, 10 mM citric acid, pH 6.0)
Sample Rat Tissue sections (Rat placenta tissue)
Specification Rat placenta tissue
Permeabilization Yes - 0.3% Triton X-100
Fixative Formaldehyde
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Submitted Feb 03 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: 23°C
Sample Mouse Cell (Mouse erythroblasts)
Specification Mouse erythroblasts
Permeabilization Yes - 0.1% Triton X-100
Fixative Paraformaldehyde
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Submitted Jan 23 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: 23°C
Sample Human Cultured Cells (Human trophoblast cell line)
Specification Human trophoblast cell line
Permeabilization Yes - 0.1% Triton X-100
Fixative Paraformaldehyde
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Submitted Jan 23 2014

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (12.5%)
Sample Human Cell lysate - whole cell (Human peripheral blood neutrophils)
Specification Human peripheral blood neutrophils
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Jan 23 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Sample Human Cell (Human peripheral blood neutrophils)
Specification Human peripheral blood neutrophils
Permeabilization Yes - 0.3% Triton X-100
Fixative Paraformaldehyde
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Submitted Oct 11 2013

Vielen Dank für Ihr Interesse an unseren Produkten.

Leider haben wir aus logistischen Gründen keine Aliquots von unseren Antikörpern, die wir Ihnen senden könnten. Ich kann Ihnen jedoch versichern, dass wir unsere Produkte garantieren so zu ...

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The theoretical weight of CD82 is around 34 kD (in reducing conditions) The band size is very difficult to determine because it migrates as a smear, due to high level of N-Glycosylation. This glycosylation could depend on the cell type and the state of...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"