Anti-CD83 antibody [HB15e] (Phycoerythrin) (ab95779)

Overview

  • Product nameAnti-CD83 antibody [HB15e] (Phycoerythrin)
    See all CD83 primary antibodies
  • Description
    Mouse monoclonal [HB15e] to CD83 (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    COS cells transfected with Human CD83.

  • Positive control
    • One day PHA stimulated normal Human peripheral blood cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab95779 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 5µl for 106 cells. ab91357-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionMay play a significant role in antigen presentation or the cellular interactions that follow lymphocyte activation.
  • Tissue specificityExpressed by activated lymphocytes, Langerhans cells and interdigitating reticulum cells.
  • Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • B cell activation 45kDa cell surface glycoprotein Ig superfamily antibody
    • B cell activation protein antibody
    • B-cell activation protein antibody
    • BL11 antibody
    • BL11 PEN antibody
    • CD83 antibody
    • CD83 antigen (activated B lymphocytes, immunoglobulin superfamily) antibody
    • CD83 antigen antibody
    • CD83 molecule antibody
    • CD83_HUMAN antibody
    • Cell surface glycoprotein antibody
    • Cell surface protein HB15 antibody
    • HB15 antibody
    • hCD83 antibody
    see all

Anti-CD83 antibody [HB15e] (Phycoerythrin) images

  • Flow cytometric analysis of 1 day PHA stimulated normal Human peripheral blood cells with Mouse IgG1 Isotype Control PE (left) or ab95779 at 0.5 µg (right).
    Cells in the lymphocyte gate were used for analysis.

References for Anti-CD83 antibody [HB15e] (Phycoerythrin) (ab95779)

ab95779 has not yet been referenced specifically in any publications.

Product Wall

Thank you very much for contacting us.

The lab has confirmed that 5 µg/ml of PHA-L has been used to stimulate the PBMC as depicted in the image (PHA-L = Lectin from Phaseolus vulgaris, red kidney bean).

As we have not stimulat...

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Thank you for contacting us.

To our knowledge, CD83, a member of the Ig superfamily, is expressed on cultured dendritic cells, interdigitating, follicular, and circulating dendritic cells as well as some proliferating lymphocytes, and human c...

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Thank you for your reply. I have sent an email to our rep that is in charge of orders that are shipped to , to have another vial of ab95779 sent to make up for the volume shortage of the original product. I have also asked if it is possible that this c...

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Thank you for your patience while I determined the actual volumes that you should have received. For ab95779, Anti-CD83 antibody [HB15e] (Phycoerythrin), you should have received a total volume of 2ml (2000ul), as you only received 15.ml (1500ul), I am...

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Thank you for calling Abcam earlier. I am sorry about all the shortages that you have been having recently with Abcam products. For ab26004 (part of ab106073), I have read through ****notes and it appears we are still waiting for confirmation from the ...

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Thank you for your email and your phone call. I can confirm that for ab95779 a volume of 2 ml should be in the vial. I checked with our shipping department and the vial you received should be an amber (brown) vial. I'd suggest passing the vial to th...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"