Anti-CD84 antibody [CD84.1.21] (Allophycocyanin), prediluted (ab176513)

Overview

  • Product name
    Anti-CD84 antibody [CD84.1.21] (Allophycocyanin), prediluted
    See all CD84 primary antibodies
  • Description
    Mouse Monoclonal [CD84.1.21] to CD84, prediluted (Allophycocyanin)
  • Conjugation
    Allophycocyanin. Ex: 645nm, Em: 660nm
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue, cells or virus corresponding to Human CD84 . (CD84-transfected 300.19 cell line).

  • Positive control
    • Human blood cells.

Properties

  • Form
    Prediluted
  • Storage instructions
    Shipped at 4°C. Store at +4°C. Store In the Dark.
  • Storage buffer
    Preservative: 0.098% Sodium azide
    Constituents: 0.2% BSA, 99% PBS
  • Concentration information loading...
  • Purity
    Size exclusion
  • Purification notes
    Purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
  • Clonality
    Monoclonal
  • Clone number
    CD84.1.21
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab176513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 10µl for 106 cells.

(or 100 μl of whole blood).

Target

  • Function
    Plays a role as adhesion receptor functioning by homophilic interactions and by clustering. Recruits SH2 domain-containing proteins SH2D1A/SAP. Increases proliferative responses of activated T-cells and SH2D1A/SAP does not seen be required for this process. Homophilic interactions enhance interferon gamma/IFNG secretion in lymphocytes and induce platelet stimulation via a SH2D1A/SAP-dependent pathway. May serve as a marker for hematopoietic progenitor cells.
  • Tissue specificity
    Predominantly expressed in hematopoietic tissues, such as lymph node, spleen and peripheral leukocytes. Expressed in macrophages, B-cells, monocytes, platelets, thymocytes, T-cells and dendritic cells. Highly expressed in memory T-cells.
  • Sequence similarities
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
  • Developmental stage
    Expression is slightly increased in naive B-cells after the first dividion. By contrast, expression on memory B-cells decreased with each successive division.
  • Domain
    ITSM (immunoreceptor tyrosine-based switch motif) motif is a cytoplasmic motif which may bind SH2D1A.
  • Post-translational
    modifications
    Phosphorylated by tyrosine-protein kinase LCK on tyrosine residues following ligation induced by agonist monoclonal antibody. The association with SH2D1A/SAP is dependent of tyrosines phosphorylation of its cytoplasmic domain Phosphorylated on Tyr-296 and Tyr-316 following platelet aggregation.
    N-glycosylated.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD_antigen=CD84 antibody
    • CD84 antibody
    • CD84 antigen antibody
    • CD84 molecule antibody
    • CDw84 antibody
    • Cell surface antigen MAX.3 antibody
    • hCD84 antibody
    • Hly9 beta antibody
    • Hly9-beta antibody
    • Leucocyte differentiation antigen CD84 antibody
    • Leukocyte Antigen antibody
    • Leukocyte antigen CD84 antibody
    • Leukocyte differentiation antigen antibody
    • Leukocyte differentiation antigen CD84 antibody
    • LY9B antibody
    • mCD84 antibody
    • Signaling lymphocytic activation molecule 5 antibody
    • SLAF5_HUMAN antibody
    • SLAM family member 5 antibody
    • SLAMF5 antibody
    see all

References

This product has been referenced in:
  • Romero X  et al. CD229 (Ly9) lymphocyte cell surface receptor interacts homophilically through its N-terminal domain and relocalizes to the immunological synapse. J Immunol 174:7033-42 (2005). Read more (PubMed: 15905546) »
  • Sayós J  et al. Cell surface receptors Ly-9 and CD84 recruit the X-linked lymphoproliferative disease gene product SAP. Blood 97:3867-74 (2001). Read more (PubMed: 11389028) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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