Anti-CD84 antibody [CD84.1.21] (Phycoerythrin) (ab33218)

Overview

  • Product name
    Anti-CD84 antibody [CD84.1.21] (Phycoerythrin)
    See all CD84 primary antibodies
  • Description
    Mouse monoclonal [CD84.1.21] to CD84 (Phycoerythrin)
  • Conjugation
    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Specificity
    Recognizes CD84 cell surface antigen, a highly glycosylated cell surface antigen of 64-82kD.
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Mouse pre-B cell line 300.19 transfected with Human CD84 cDNA.

  • Positive control
    • Peripheral blood monocytes
  • General notes

    Purified IgG conjugated to R. Phycoerythrin (RPE).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    CD84.1.21
  • Myeloma
    NS1
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab33218 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 10µl for 106 cells. or 100µl whole blood.

Target

  • Function
    Plays a role as adhesion receptor functioning by homophilic interactions and by clustering. Recruits SH2 domain-containing proteins SH2D1A/SAP. Increases proliferative responses of activated T-cells and SH2D1A/SAP does not seen be required for this process. Homophilic interactions enhance interferon gamma/IFNG secretion in lymphocytes and induce platelet stimulation via a SH2D1A/SAP-dependent pathway. May serve as a marker for hematopoietic progenitor cells.
  • Tissue specificity
    Predominantly expressed in hematopoietic tissues, such as lymph node, spleen and peripheral leukocytes. Expressed in macrophages, B-cells, monocytes, platelets, thymocytes, T-cells and dendritic cells. Highly expressed in memory T-cells.
  • Sequence similarities
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
  • Developmental stage
    Expression is slightly increased in naive B-cells after the first dividion. By contrast, expression on memory B-cells decreased with each successive division.
  • Domain
    ITSM (immunoreceptor tyrosine-based switch motif) motif is a cytoplasmic motif which may bind SH2D1A.
  • Post-translational
    modifications
    Phosphorylated by tyrosine-protein kinase LCK on tyrosine residues following ligation induced by agonist monoclonal antibody. The association with SH2D1A/SAP is dependent of tyrosines phosphorylation of its cytoplasmic domain Phosphorylated on Tyr-296 and Tyr-316 following platelet aggregation.
    N-glycosylated.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD_antigen=CD84 antibody
    • CD84 antibody
    • CD84 antigen antibody
    • CD84 molecule antibody
    • CDw84 antibody
    • Cell surface antigen MAX.3 antibody
    • hCD84 antibody
    • Hly9 beta antibody
    • Hly9-beta antibody
    • Leucocyte differentiation antigen CD84 antibody
    • Leukocyte Antigen antibody
    • Leukocyte antigen CD84 antibody
    • Leukocyte differentiation antigen antibody
    • Leukocyte differentiation antigen CD84 antibody
    • LY9B antibody
    • mCD84 antibody
    • Signaling lymphocytic activation molecule 5 antibody
    • SLAF5_HUMAN antibody
    • SLAM family member 5 antibody
    • SLAMF5 antibody
    see all

Images

  • Staining of human peripheral blood monocytes with RPE-conjugated anti-CD84 (ab33218)

References

ab33218 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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