Overview

  • Product nameAnti-CD84 antibody [mCD84.7] (Allophycocyanin)
    See all CD84 primary antibodies
  • Description
    Armenian Hamster monoclonal [mCD84.7] to CD84 (Allophycocyanin)
  • ConjugationAllophycocyanin. Ex: 645nm, Em: 660nm
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Mouse CD84-Fc fusion protein

  • Positive control
    • Splenocytes or bone marrow

Properties

Applications

Our Abpromise guarantee covers the use of ab95696 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.5-1µg for 105-8 cells. Proceed to staining in a final volume of 100µl.

Target

  • FunctionPlays a role as adhesion receptor functioning by homophilic interactions and by clustering. Recruits SH2 domain-containing proteins SH2D1A/SAP. Increases proliferative responses of activated T-cells and SH2D1A/SAP does not seen be required for this process. Homophilic interactions enhance interferon gamma/IFNG secretion in lymphocytes and induce platelet stimulation via a SH2D1A/SAP-dependent pathway. May serve as a marker for hematopoietic progenitor cells.
  • Tissue specificityPredominantly expressed in hematopoietic tissues, such as lymph node, spleen and peripheral leukocytes. Expressed in macrophages, B-cells, monocytes, platelets, thymocytes, T-cells and dendritic cells. Highly expressed in memory T-cells.
  • Sequence similaritiesContains 1 Ig-like C2-type (immunoglobulin-like) domain.
  • Developmental stageExpression is slightly increased in naive B-cells after the first dividion. By contrast, expression on memory B-cells decreased with each successive division.
  • DomainITSM (immunoreceptor tyrosine-based switch motif) motif is a cytoplasmic motif which may bind SH2D1A.
  • Post-translational
    modifications
    Phosphorylated by tyrosine-protein kinase LCK on tyrosine residues following ligation induced by agonist monoclonal antibody. The association with SH2D1A/SAP is dependent of tyrosines phosphorylation of its cytoplasmic domain Phosphorylated on Tyr-296 and Tyr-316 following platelet aggregation.
    N-glycosylated.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD_antigen=CD84 antibody
    • CD84 antibody
    • CD84 antigen antibody
    • CD84 molecule antibody
    • CDw84 antibody
    • Cell surface antigen MAX.3 antibody
    • hCD84 antibody
    • Hly9 beta antibody
    • Hly9-beta antibody
    • Leucocyte differentiation antigen CD84 antibody
    • Leukocyte Antigen antibody
    • Leukocyte antigen CD84 antibody
    • Leukocyte differentiation antigen antibody
    • Leukocyte differentiation antigen CD84 antibody
    • LY9B antibody
    • mCD84 antibody
    • Signaling lymphocytic activation molecule 5 antibody
    • SLAF5_HUMAN antibody
    • SLAM family member 5 antibody
    • SLAMF5 antibody
    see all

Anti-CD84 antibody [mCD84.7] (Allophycocyanin) images

  • Lineage C57BL/6 murine bone marrow cells were stained with anti-Ly-6A/E (Sca-1) PE-conjugated and 0.5µg of ab95696.
  • Staining of mouse splenocytes with 0.5 µg of Allophycocyanin-conjugated Armenian Hamster IgG isotype control (blue histogram) or 0.5 µg of ab95696 (purple histogram).
    Total viable cells were used for analysis.

References for Anti-CD84 antibody [mCD84.7] (Allophycocyanin) (ab95696)

This product has been referenced in:
  • Romero X  et al. CD229 (Ly9) lymphocyte cell surface receptor interacts homophilically through its N-terminal domain and relocalizes to the immunological synapse. J Immunol 174:7033-42 (2005). Flow Cyt ; Mouse . Read more (PubMed: 15905546) »

See 1 Publication for this product

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