The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesBL:In vivo and in vitro blocking assays: Use at an assay dependent dilution.
Flow Cyt: Use 1µg for 106 cells.
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionReceptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.
Tissue specificityExpressed by activated B-lymphocytes and monocytes.
BALB/c spleen cells were activated by incubation for 72 hours with LPS. The cells were then harvested and stained with ab25143 (at a concentration of 1µg/106 cells) . A wide gate was set to include small, medium and large cells, following which the cells were analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA).
References for Anti-CD86 antibody [2D10] (FITC) (ab25143)
has not yet been referenced specifically in any publications.
Publishing research using ab25143? Please let us know so that we can cite the reference in this datasheet.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"