• Product nameAnti-CD86 antibody [2D10] (FITC)
    See all CD86 primary antibodies
  • Description
    Rat monoclonal [2D10] to CD86 (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Specificityab25143 recognises CD86, the B7-2 costimulatory molecule and a ligand for CD28 and CD152.
  • Tested applicationsSuitable for: Flow Cyt, IHC-Fr, IP, Blockingmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    The details of the immunogen for this antibody are not available.



Our Abpromise guarantee covers the use of ab25143 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notesBL:In vivo and in vitro blocking assays: Use at an assay dependent dilution.
    Flow Cyt: Use 1µg for 106 cells.
    IHC-Fr: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionReceptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.
    • Tissue specificityExpressed by activated B-lymphocytes and monocytes.
    • Sequence similaritiesContains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Post-translational
      Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Activation B7-2 antigen 3 antibody
      • Activation B7-2 antigen antibody
      • B-lymphocyte activation antigen B7-2 2 antibody
      • B-lymphocyte activation antigen B7-2 antibody
      • B7 2 antibody
      • B7 antibody
      • B7-2 antibody
      • B7.2 antibody
      • B70 antibody
      • B72 antigen antibody
      • BU63 antibody
      • CD28 antigen ligand 2 2 antibody
      • CD28 antigen ligand 2 antibody
      • Cd28l2 antibody
      • CD28LG2 antibody
      • CD86 antibody
      • CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) 1, 2 antibody
      • CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) antibody
      • CD86 antigen antibody
      • CD86 molecule antibody
      • CD86_HUMAN antibody
      • CLS1 antibody
      • CTLA-4 counter-receptor B7.2 2, 3 antibody
      • CTLA-4 counter-receptor B7.2 antibody
      • Early T-cell costimulatory molecule 1 antibody
      • ETC-1 antibody
      • FUN 1 antibody
      • FUN-1 antibody
      • LAB72 antibody
      • Ly-58 antibody
      • Ly58 antibody
      • MB7 antibody
      • MB7-2 antibody
      • Membrane glycoprotein antibody
      • MGC34413 antibody
      • T-lymphocyte activation antigen CD86 antibody
      • TS/A-2 antibody
      see all

    Anti-CD86 antibody [2D10] (FITC) images

    • BALB/c spleen cells were activated by incubation for 72 hours with LPS. The cells were then harvested and stained with ab25143 (at a concentration of 1µg/106 cells) . A wide gate was set to include small, medium and large cells, following which the cells were analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA).

    References for Anti-CD86 antibody [2D10] (FITC) (ab25143)

    ab25143 has not yet been referenced specifically in any publications.

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