Overview

  • Product nameAnti-CD86 antibody [BU63] (FITC)
    See all CD86 primary antibodies
  • Description
    Mouse monoclonal [BU63] to CD86 (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    B-lymphoblastoid cell line ARH 77

  • Positive control
    • Human blood cells.
  • General notes

    The antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use.

Properties

Applications

Our Abpromise guarantee covers the use of ab77276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notesFlow Cyt: Use 20µl for 106 cells or 100µl whole blood.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionReceptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.
    • Tissue specificityExpressed by activated B-lymphocytes and monocytes.
    • Sequence similaritiesContains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Post-translational
      modifications
      Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Activation B7-2 antigen 3 antibody
      • Activation B7-2 antigen antibody
      • B-lymphocyte activation antigen B7-2 2 antibody
      • B-lymphocyte activation antigen B7-2 antibody
      • B7 2 antibody
      • B7 antibody
      • B7-2 antibody
      • B7.2 antibody
      • B70 antibody
      • B72 antigen antibody
      • BU63 antibody
      • CD28 antigen ligand 2 2 antibody
      • CD28 antigen ligand 2 antibody
      • Cd28l2 antibody
      • CD28LG2 antibody
      • CD86 antibody
      • CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) 1, 2 antibody
      • CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) antibody
      • CD86 antigen antibody
      • CD86 molecule antibody
      • CD86_HUMAN antibody
      • CLS1 antibody
      • CTLA-4 counter-receptor B7.2 2, 3 antibody
      • CTLA-4 counter-receptor B7.2 antibody
      • Early T-cell costimulatory molecule 1 antibody
      • ETC-1 antibody
      • FUN 1 antibody
      • FUN-1 antibody
      • LAB72 antibody
      • Ly-58 antibody
      • Ly58 antibody
      • MB7 antibody
      • MB7-2 antibody
      • Membrane glycoprotein antibody
      • MGC34413 antibody
      • T-lymphocyte activation antigen CD86 antibody
      • TS/A-2 antibody
      see all

    References for Anti-CD86 antibody [BU63] (FITC) (ab77276)

    This product has been referenced in:
    • Giguère JF  et al. Insertion of host-derived costimulatory molecules CD80 (B7.1) and CD86 (B7.2) into human immunodeficiency virus type 1 affects the virus life cycle. J Virol 78:6222-32 (2004). Read more (PubMed: 15163715) »
    • Mauri D  et al. Antigen-presenting T cells induce the development of cytotoxic CD4+ T cells. I. Involvement of the CD80-CD28 adhesion molecules. J Immunol 155:118-27 (1995). Read more (PubMed: 7541409) »

    See all 4 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"