Overview

  • Product name
    Anti-CD86 antibody [BU63] (PerCP)
    See all CD86 primary antibodies
  • Description
    Mouse monoclonal [BU63] to CD86 (PerCP)
  • Host species
    Mouse
  • Conjugation
    PerCP. Ex: 482nm, Em: 675nm
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    B-lymphoblastoid cell line ARH 77

  • Positive control
    • Human peripheral blood cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab77131 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notes
    Flow Cyt: Use 10 µl for 106 cells or 100 µl whole blood.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Function
      Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.
    • Tissue specificity
      Expressed by activated B-lymphocytes and monocytes.
    • Sequence similarities
      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Post-translational
      modifications
      Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
      • Alternative names
        • Activation B7-2 antigen 3 antibody
        • Activation B7-2 antigen antibody
        • B-lymphocyte activation antigen B7-2 2 antibody
        • B-lymphocyte activation antigen B7-2 antibody
        • B7 2 antibody
        • B7 antibody
        • B7-2 antibody
        • B7.2 antibody
        • B70 antibody
        • B72 antigen antibody
        • BU63 antibody
        • CD28 antigen ligand 2 2 antibody
        • CD28 antigen ligand 2 antibody
        • Cd28l2 antibody
        • CD28LG2 antibody
        • CD86 antibody
        • CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) 1, 2 antibody
        • CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) antibody
        • CD86 antigen antibody
        • CD86 molecule antibody
        • CD86_HUMAN antibody
        • CLS1 antibody
        • CTLA-4 counter-receptor B7.2 2, 3 antibody
        • CTLA-4 counter-receptor B7.2 antibody
        • Early T-cell costimulatory molecule 1 antibody
        • ETC-1 antibody
        • FUN 1 antibody
        • FUN-1 antibody
        • LAB72 antibody
        • Ly-58 antibody
        • Ly58 antibody
        • MB7 antibody
        • MB7-2 antibody
        • Membrane glycoprotein antibody
        • MGC34413 antibody
        • T-lymphocyte activation antigen CD86 antibody
        • TS/A-2 antibody
        see all

      Images

      • Flow Cytometry - Anti-CD86 antibody [BU63] (PerCP) (ab77131)
        Flow Cytometry - Anti-CD86 antibody [BU63] (PerCP) (ab77131)

        ab77131 staining, surface CD86 in human peripheral blood cells by Flow Cytometery. Monocytes were used for gating. The blue peak reperesents negative control while red peak show staining with ab77131.

      References

      ab77131 has not yet been referenced specifically in any publications.

      Publishing research using ab77131? Please let us know so that we can cite the reference in this datasheet.

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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