Anti-CD86 antibody [BU63] (PerCP) (ab77131)

Overview

• Product name
  Anti-CD86 antibody [BU63] (PerCP)
  See all CD86 primary antibodies
• Description
  Mouse monoclonal [BU63] to CD86 (PerCP)
• Conjugation
  PerCP. Ex: 482nm, Em: 675nm
• Tested applications
  Suitable for: Flow Cytmore details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  B-lymphoblastoid cell line ARH 77

• Positive control
  • Human peripheral blood cells.

Properties

• Form
  Liquid
• Storage instructions
  Shipped at 4°C. Store at +4°C.
• Storage buffer
  Preservative: 15mM Sodium Azide
  Constituents: 0.2% BSA (high-grade protease free), PBS
• Concentration information loading...
• Purity
  Size exclusion
• Purification notes
  ab77131 is purified by size exclusion chromatography and adjusted for direct use.
• Clonality
  Monoclonal
• Clone number
  BU63
• Isotype
  IgG1
• Research areas
  • Immunology
  • Adaptive Immunity
  • T Cells
  • CD

  • Immunology
  • Cell Type Markers
  • CD
  • Non-lineage

  • Microbiology
  • Interspecies Interaction
  • Host Virus Interaction

  • Stem Cells
  • Hematopoietic Progenitors
  • Lymphoid
  • B Lymphocytic Lineage

  • Stem Cells
  • Hematopoietic Progenitors
  • Myeloid
  • Dendritic Cell Lineage

  • Stem Cells
  • Hematopoietic Progenitors
  • Myeloid
  • Monocytic Lineage

  • Immunology
  • Adaptive Immunity
  • Regulatory T Cells

  • Kits/ Lysates/ Other
  • Kits
  • ELISA Kits
  • ELISA Kits
  • CD markers ELISA kits

Associated products

• Compatible Secondaries
  • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
  • Goat Anti-Mouse IgG H&L (HRP) (ab205719)

Applications

Target

• Function
  Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.
• Tissue specificity
  Expressed by activated B-lymphocytes and monocytes.
• Sequence similarities
  Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
  Contains 1 Ig-like V-type (immunoglobulin-like) domain.
• Post-translational
  modifications
  Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.
• Cellular localization
  Membrane.

• Target information above from: UniProt accession P42081 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• Database links
  • Entrez Gene: 942 Human
  • Omim: 601020 Human
  • SwissProt: P42081 Human
  • Unigene: 171182 Human
• Alternative names
  • Activation B7-2 antigen 3 antibody
  • Activation B7-2 antigen antibody
  • B-lymphocyte activation antigen B7-2 2 antibody

  • B-lymphocyte activation antigen B7-2 antibody
  • B7 2 antibody
  • B7 antibody
  • B7-2 antibody
  • B7.2 antibody
  • B70 antibody
  • B72 antigen antibody
  • BU63 antibody
  • CD28 antigen ligand 2 2 antibody
  • CD28 antigen ligand 2 antibody
  • Cd28l2 antibody
  • CD28LG2 antibody
  • CD86 antibody
  • CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) 1, 2 antibody
  • CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) antibody
  • CD86 antigen antibody
  • CD86 molecule antibody
  • CD86_HUMAN antibody
  • CLS1 antibody
  • CTLA-4 counter-receptor B7.2 2, 3 antibody
  • CTLA-4 counter-receptor B7.2 antibody
  • Early T-cell costimulatory molecule 1 antibody
  • ETC-1 antibody
  • FUN 1 antibody
  • FUN-1 antibody
  • LAB72 antibody
  • Ly-58 antibody
  • Ly58 antibody
  • MB7 antibody
  • MB7-2 antibody
  • Membrane glycoprotein antibody
  • MGC34413 antibody
  • T-lymphocyte activation antigen CD86 antibody
  • TS/A-2 antibody

  see all

Protocols

• Flow cytometry protocols