Overview

  • Product nameAnti-CD86 antibody [BU63] (Phycoerythrin)
    See all CD86 primary antibodies
  • Description
    Mouse monoclonal [BU63] to CD86 (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • Specificityab77226 reacts with CD86 expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    B-lymphoblastoid cell line ARH 77

  • General notes

    The antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions.

Properties

Applications

Our Abpromise guarantee covers the use of ab77226 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notesFlow Cyt: The reagent is designed for Flow Cytometry analysis of human blood cells
    Use 20 µl/100 µl of whole blood or 106 cells in a suspension.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionReceptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.
    • Tissue specificityExpressed by activated B-lymphocytes and monocytes.
    • Sequence similaritiesContains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Post-translational
      modifications
      Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Activation B7-2 antigen 3 antibody
      • Activation B7-2 antigen antibody
      • B-lymphocyte activation antigen B7-2 2 antibody
      • B-lymphocyte activation antigen B7-2 antibody
      • B7 2 antibody
      • B7 antibody
      • B7-2 antibody
      • B7.2 antibody
      • B70 antibody
      • B72 antigen antibody
      • BU63 antibody
      • CD28 antigen ligand 2 2 antibody
      • CD28 antigen ligand 2 antibody
      • Cd28l2 antibody
      • CD28LG2 antibody
      • CD86 antibody
      • CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) 1, 2 antibody
      • CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) antibody
      • CD86 antigen antibody
      • CD86 molecule antibody
      • CD86_HUMAN antibody
      • CLS1 antibody
      • CTLA-4 counter-receptor B7.2 2, 3 antibody
      • CTLA-4 counter-receptor B7.2 antibody
      • Early T-cell costimulatory molecule 1 antibody
      • ETC-1 antibody
      • FUN 1 antibody
      • FUN-1 antibody
      • LAB72 antibody
      • Ly-58 antibody
      • Ly58 antibody
      • MB7 antibody
      • MB7-2 antibody
      • Membrane glycoprotein antibody
      • MGC34413 antibody
      • T-lymphocyte activation antigen CD86 antibody
      • TS/A-2 antibody
      see all

    Anti-CD86 antibody [BU63] (Phycoerythrin) images

    • Flow cytometry analysis of human peripheral blood leukocytes with anti-human CD86 (BU63) PE. 

    References for Anti-CD86 antibody [BU63] (Phycoerythrin) (ab77226)

    This product has been referenced in:
    • Giguère JF  et al. Insertion of host-derived costimulatory molecules CD80 (B7.1) and CD86 (B7.2) into human immunodeficiency virus type 1 affects the virus life cycle. J Virol 78:6222-32 (2004). Read more (PubMed: 15163715) »
    • Mauri D  et al. Antigen-presenting T cells induce the development of cytotoxic CD4+ T cells. I. Involvement of the CD80-CD28 adhesion molecules. J Immunol 155:118-27 (1995). Read more (PubMed: 7541409) »

    See all 4 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"