The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesFlow Cyt: The reagent is designed for Flow Cytometry analysis of human blood cells
Use 20 µl/100 µl of whole blood or 106 cells in a suspension.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionReceptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.
Tissue specificityExpressed by activated B-lymphocytes and monocytes.
Flow cytometry analysis of human peripheral blood leukocytes with anti-human CD86 (BU63) PE.
References for Anti-CD86 antibody [BU63] (Phycoerythrin) (ab77226)
This product has been referenced in:
Giguère JF et al. Insertion of host-derived costimulatory molecules CD80 (B7.1) and CD86 (B7.2) into human immunodeficiency virus type 1 affects the virus life cycle. J Virol78:6222-32 (2004).
Read more (PubMed: 15163715) »
Mauri D et al. Antigen-presenting T cells induce the development of cytotoxic CD4+ T cells. I. Involvement of the CD80-CD28 adhesion molecules. J Immunol155:118-27 (1995).
Read more (PubMed: 7541409) »