Anti-CD86 antibody [GL-1] (ab119857)
Key features and details
- Rat monoclonal [GL-1] to CD86
- Suitable for: IHC-Fr, Blocking, IP, ICC/IF, Functional Studies, Flow Cyt
- Reacts with: Mouse
- Isotype: IgG2a
Related conjugates and formulations
Overview
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Product name
Anti-CD86 antibody [GL-1]
See all CD86 primary antibodies -
Description
Rat monoclonal [GL-1] to CD86 -
Host species
Rat -
Tested applications
Suitable for: IHC-Fr, Blocking, IP, ICC/IF, Functional Studies, Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
LPS-activated CBA/Cs Mouse splenic B cells.
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Positive control
- Mouse splenocytes.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. Store undiluted. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.87% Sodium chloride, 0.14% Monobasic dihydrogen sodium phosphate -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
This purified format is guaranteed to be >90% pure as determined by SDS-PAGE analysis. -
Clonality
Monoclonal -
Clone number
GL-1 -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab119857 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
Use at an assay dependent concentration.
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Blocking |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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Functional Studies |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Notes |
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IHC-Fr
Use at an assay dependent concentration. |
Blocking
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
Functional Studies
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Target
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Function
Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. -
Tissue specificity
Expressed by activated B-lymphocytes and monocytes. -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsPolyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 12524 Mouse
- SwissProt: P42082 Mouse
- Unigene: 1452 Mouse
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Alternative names
- Activation B7-2 antigen 3 antibody
- Activation B7-2 antigen antibody
- B-lymphocyte activation antigen B7-2 2 antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (27)
ab119857 has been referenced in 27 publications.
- Zhou J et al. Single-cell RNA sequencing to characterize the response of pancreatic cancer to anti-PD-1 immunotherapy. Transl Oncol 15:101262 (2022). PubMed: 34768100
- Zhou T et al. miR-204-containing exosomes ameliorate GVHD-associated dry eye disease. Sci Adv 8:eabj9617 (2022). PubMed: 35020440
- Ma Y et al. Photobiomodulation Attenuates Neurotoxic Polarization of Macrophages by Inhibiting the Notch1-HIF-1α/NF-κB Signalling Pathway in Mice With Spinal Cord Injury. Front Immunol 13:816952 (2022). PubMed: 35371065
- Li H et al. Notoginsenoside R1-loaded mesoporous silica nanoparticles targeting the site of injury through inflammatory cells improves heart repair after myocardial infarction. Redox Biol 54:102384 (2022). PubMed: 35777198
- Li Y et al. P2X7R-NEK7-NLRP3 Inflammasome Activation: A Novel Therapeutic Pathway of Qishen Granule in the Treatment of Acute Myocardial Ischemia. J Inflamm Res 15:5309-5326 (2022). PubMed: 36124207