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Tissue/ cell preparation (Mouse): LPS-activated B cells.
Our Abpromise guarantee covers the use of ab24862 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Blocking||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.
ab18446-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|IHC-Fr||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
Flow Cytometry analysis of C57Bl/6 splenocytes unstimulated (left panel) or stimulated for 3 days with LPS (right panel) labeling CD86 with ab24862 at 0.125µg (solid line). Rat IgG2a (FITC) isotype control 0.125µg (dotted line).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"