Overview

  • Product nameAnti-CD86 antibody [GL1] (PE/Cy7 ®)
    See all CD86 primary antibodies
  • Description
    Rat monoclonal [GL1] to CD86 (PE/Cy7 ®)
  • ConjugationPE/Cy7 ®. Ex: 496nm, Em: 774nm
  • Tested applicationsSuitable for: Flow Cyt, IHC-Fr, Blocking, IPmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    LPS activated mouse B cells

  • Positive control
    • Flow Cyt: BALB/c spleen cells activated by incubation for 72 hours with LPS.
  • General notesThis product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.

Properties

Applications

Our Abpromise guarantee covers the use of ab81995 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.1µg for 106 cells.

ab154460 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration.
Blocking Use at an assay dependent concentration. (In vivo and in vitro assays).
IP Use at an assay dependent concentration.

Target

  • FunctionReceptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.
  • Tissue specificityExpressed by activated B-lymphocytes and monocytes.
  • Sequence similaritiesContains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activation B7-2 antigen 3 antibody
    • Activation B7-2 antigen antibody
    • B-lymphocyte activation antigen B7-2 2 antibody
    • B-lymphocyte activation antigen B7-2 antibody
    • B7 2 antibody
    • B7 antibody
    • B7-2 antibody
    • B7.2 antibody
    • B70 antibody
    • B72 antigen antibody
    • BU63 antibody
    • CD28 antigen ligand 2 2 antibody
    • CD28 antigen ligand 2 antibody
    • Cd28l2 antibody
    • CD28LG2 antibody
    • CD86 antibody
    • CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) 1, 2 antibody
    • CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) antibody
    • CD86 antigen antibody
    • CD86 molecule antibody
    • CD86_HUMAN antibody
    • CLS1 antibody
    • CTLA-4 counter-receptor B7.2 2, 3 antibody
    • CTLA-4 counter-receptor B7.2 antibody
    • Early T-cell costimulatory molecule 1 antibody
    • ETC-1 antibody
    • FUN 1 antibody
    • FUN-1 antibody
    • LAB72 antibody
    • Ly-58 antibody
    • Ly58 antibody
    • MB7 antibody
    • MB7-2 antibody
    • Membrane glycoprotein antibody
    • MGC34413 antibody
    • T-lymphocyte activation antigen CD86 antibody
    • TS/A-2 antibody
    see all

References for Anti-CD86 antibody [GL1] (PE/Cy7 ®) (ab81995)

This product has been referenced in:
  • Hathcock KS  et al. Comparative analysis of B7-1 and B7-2 costimulatory ligands: expression and function. J Exp Med 180:631-40 (1994). Read more (PubMed: 7519245) »
  • Inaba K  et al. The tissue distribution of the B7-2 costimulator in mice: abundant expression on dendritic cells in situ and during maturation in vitro. J Exp Med 180:1849-60 (1994). Read more (PubMed: 7525841) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (Spleen cell)
Permeabilization No
Gating Strategy CD45+ CD11c+ CD86+
Specification Spleen cell
Preparation Cell harvesting/tissue preparation method: Smush spleens through 70um filter, then lyse RBCs
Sample buffer: PBS + 2% FBS
Fixation Formaldehyde
Username

Ms. Cheryl Sandoval

Verified customer

Submitted Jun 28 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"