Overview

  • Product nameAnti-CD86 antibody [GL1] (Phycoerythrin)
    See all CD86 primary antibodies
  • Description
    Rat monoclonal [GL1] to CD86 (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • Specificityab25284 recognises mouse CD86, the B7-2 co-stimulatory molecule and a ligand for CD28 and CD152/CTLA-4
  • Tested applicationsSuitable for: Flow Cyt, Blocking, IHC-Fr, IPmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    LPS-activated mouse B cells.

  • General notes


    ab25284 blocks mixed lymphocyte reactions and stimulation of T cells by antigen-presenting cells both in vivo and in vitro.

Properties

Applications

Our Abpromise guarantee covers the use of ab25284 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.1µg for 106 cells. ab134674-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
Blocking Use at an assay dependent concentration.

In vivo and in vitro.

IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • FunctionReceptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.
  • Tissue specificityExpressed by activated B-lymphocytes and monocytes.
  • Sequence similaritiesContains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activation B7-2 antigen 3 antibody
    • Activation B7-2 antigen antibody
    • B-lymphocyte activation antigen B7-2 2 antibody
    • B-lymphocyte activation antigen B7-2 antibody
    • B7 2 antibody
    • B7 antibody
    • B7-2 antibody
    • B7.2 antibody
    • B70 antibody
    • B72 antigen antibody
    • BU63 antibody
    • CD28 antigen ligand 2 2 antibody
    • CD28 antigen ligand 2 antibody
    • Cd28l2 antibody
    • CD28LG2 antibody
    • CD86 antibody
    • CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) 1, 2 antibody
    • CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) antibody
    • CD86 antigen antibody
    • CD86 molecule antibody
    • CD86_HUMAN antibody
    • CLS1 antibody
    • CTLA-4 counter-receptor B7.2 2, 3 antibody
    • CTLA-4 counter-receptor B7.2 antibody
    • Early T-cell costimulatory molecule 1 antibody
    • ETC-1 antibody
    • FUN 1 antibody
    • FUN-1 antibody
    • LAB72 antibody
    • Ly-58 antibody
    • Ly58 antibody
    • MB7 antibody
    • MB7-2 antibody
    • Membrane glycoprotein antibody
    • MGC34413 antibody
    • T-lymphocyte activation antigen CD86 antibody
    • TS/A-2 antibody
    see all

Anti-CD86 antibody [GL1] (Phycoerythrin) images

  • Flow cytometry analysis staining CD86 in mouse mastrocytoma cell line P815 using ab25284 at a dilution of 0.1 ug/106 cells. Rat Anti-Mouse CD86-PE was used as an isotype control.

References for Anti-CD86 antibody [GL1] (Phycoerythrin) (ab25284)

This product has been referenced in:
  • Inaba K  et al. The tissue distribution of the B7-2 costimulator in mice: abundant expression on dendritic cells in situ and during maturation in vitro. J Exp Med 180:1849-60 (1994). Read more (PubMed: 7525841) »
  • Freeman GJ  et al. Uncovering of functional alternative CTLA-4 counter-receptor in B7-deficient mice. Science 262:907-9 (1993). Read more (PubMed: 7694362) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"